血清游离轻链不同定量检测方法的应用研究进展

Advances in the Application of Different Quantitative Detection Methods For Serum Free Light Chain

血清游离轻链(serum free light chain, sFLC)是浆细胞异常增生的标志物,其水平的升高是肿瘤负荷及疾病变化的重要依据,国际骨髓瘤工作组(International Myeloma Working Group, IMWG)已将sFLC纳入浆细胞肿瘤诊断的最新指南。目前定量检测sFLC的方法主要有5种,包括Freelite■试验(英国Binding Site公司)、血清游离轻链酶联免疫吸附试验(法国西比亚公司)、血清游离轻链测定试验(美国Diazyme公司)、N Latex血清游离轻链测定试验(德国西门子公司)以及Seralite■试验(法国西比亚公司),但在患者的随访中以上方法不可交替使用,且国际上目前尚未明确指示应选用何种方法。因此,认识不同检测方法的性能、优缺点及其在疾病中的应用尤为重要。

Serum free light chains(sFLC) are markers of plasma cell dysplasia, and their elevation is an important basis for tumor load and disease changes. The International Myeloma Working Group(IMWG) has included sFLC in the latest guidelines for the diagnosis of plasma cell tumors. At present, there are five main methods for the determination of sFLC, including Freelite■ assay(The Binding Site Group Ltd,Brimingham,UK), sFLC ELISA assay(Sebia,Evry,France), sFLC assay(Diazyme Laboratories Inc., Poway CA, USA), N Latex sFLC assay(Siemens Healthineers Diagnostics GmbH, Marburg, Germany), and Seralite■ assay(Abingdon Health-Sebia,Evry, France). However, many studies have shown that the above methods cannot be used interchangeantly in the follow-up of patients, and there is no clear indication on which method should be used internationally.Therefore, it is particularly important to know the performance, advantages and disadvantages of different detection methods and their application in diseases.