男性不育症患者支原体感染与精液常规参数、精子DNA完整性的相关性研究

Correlation between mycoplasma infection and routine semen parameters,sperm DNA integrity in male infertile patients

目的探讨男性不育症患者支原体感染与精液常规参数及精子DNA完整性的关系。方法收集2019年1月至12月于本院不孕不育科门诊就诊的男性不育症患者90例为研究对象,同期另选择正常生育的男性60例作为正常对照。两组取精液进行解脲支原体(UU)培养及精液常规检测,包括精液pH值、精子浓度、精子活力、精子活率等;对男性不育症患者行精子DNA碎片检测。分析男性不育症患者UU感染与精液常规参数及精子DNA完整性的关系。结果男性不育症组和正常对照组UU感染阳性率分别为40.0%(36/90)、5.0%(3/60),差异有统计学意义(χ^2=21.138,P=0.000)。男性不育症组精液pH值、精子浓度与正常对照组比较,差异无统计学意义(均P>0.05);而精子活力、精子活率均显著低于正常对照组(均P<0.05)。男性不育症患者中,UU感染阳性组精液pH值、精子浓度与UU感染阴性组比较,差异无统计学意义(均P>0.05);而精子活力、精子活率均显著低于UU感染阴性组,精子DNA碎片率高于UU感染阴性组(均P<0.05)。男性不育症患者UU感染与精子活力、精子活率呈负相关,与精子DNA碎片率呈正相关(rs=-0.341、-0.327、0.398,均P<0.05)。结论男性不育症患者UU感染与精子活力、精子活率及精子DNA完整性有关,UU感染可影响男性精子质量和精子DNA完整性。

Objective To investigate the correlation between mycoplasma infection and routine semen parameters,sperm DNA integrity in male infertile patients.Methods Included in this study were 90 male infertile patients who were treated in the infertility clinic of our hospital between January and December 2019(infertility group)and a contemporary cohort of 60 normal fertile males(normal control group).Semen samples of the both groups were collected for detection of Ureaplasma urealyticum(UU),and measurements of semen pH,density,vitality and motility.Male infertile patients received testing of sperm DNA fragmentation.The correlation between UU infection and routine semen parameters,sperm DNA integrity was analyzed.Results The positive rate of UU infection in the male infertile group(40.0%,36/90)was significantly higher than that in the normal control group(5.0%,3/60)(χ2=21.138,P=0.000).There was no significant difference in semen pH or sperm density between the two groups(both P>0.05).Sperm vitality and sperm motility in the male infertile group were lower than those in the normal control group(both P<0.05).Among male infertile patients,there was no significant difference in semen pH or sperm density between the UU-positive and UU-negative semen samples(both P>0.05).However,compared with UU-negative semen samples,the UU-positive semen samples showed lower sperm vitality and sperm motility,and higher rate of sperm DNA fragmentation rate(all P<0.05).In male infertile patients,UU infection was negatively correlated with sperm vitality and sperm motility,but positively correlated with sperm DNA fragmentation rate(rs=−0.341,−0.327 and 0.398,all P<0.05).Conclusion UU infection in male infertile patients is related to sperm vitality,sperm motility and sperm DNA integrity.UU infection may affect male sperm quality and sperm DNA integrity.