鹰嘴豆肽对免疫低下小鼠免疫功能的影响

Effect of Chickpea Peptide on Immune Function of Immunocompromised Mice

目的:研究鹰嘴豆肽对免疫低下小鼠免疫功能的影响。方法:60只雄性ICR小鼠按体质量随机分为空白组、环磷酰胺模型组以及鹰嘴豆肽低、中、高剂量组,每组12只。实验第1~3天,除空白组外,其余4组每天腹腔注射80 mg/kg m_b环磷酰胺,建立免疫低下小鼠模型。随后每天鹰嘴豆肽低、中、高剂量组分别灌胃0.4、0.8、1.6 g/kg m_b鹰嘴豆肽水溶液,空白组和环磷酰胺模型组动物灌胃1.6 g/kg m_b酪蛋白,连续灌胃15 d后测定小鼠体质量、脾脏和胸腺质量及形态、白细胞计数、免疫球蛋白含量、外周血T淋巴细胞亚群、细胞因子水平、骨髓有核细胞计数和DNA质量浓度。结果:造模后,小鼠脾脏和胸腺镜下形态学结构紊乱,白细胞计数,CD3^+、CD4^+、CD8^+淋巴细胞比例,免疫球蛋白质量浓度,骨髓有核细胞浓度和DNA质量浓度等显著降低(P<0.05),细胞因子水平升高(P<0.05);相比于模型组,鹰嘴豆肽干预组小鼠上述指标有不同程度的改善,其中以中、高剂量组效果最为显著(P<0.05)。结论:鹰嘴豆肽可以增强环磷酰胺造成的免疫低下小鼠的免疫功能。

Objective:To explore the effect of chickpea peptide on the immune function in immunocompromised mice.Methods:Sixty male ICR mice were randomly divided into control,cyclophosphamide model and low-,medium-and high-dose chickpea peptide groups with 12 mice in each group.In the first three days of the experiment,the mice in all groups except the control group were intraperitoneally injected with cyclophosphamide at a daily dose of 80 mg/kg mb to establish an immunocompromised mouse model.Subsequently,the mice in the low-,medium-and high-dose groups were administered with chickpea peptide at doses of 0.4,0.8 and 1.6 g/kg mb and those in the control and cyclophosphamide model groups with casein at 1.6 g/kg mb.After continuous gavage for 15 consecutive days,body mass,the mass and morphology of spleen and thymus,white blood cell count,immunoglobulin contents,peripheral blood T lymphocyte subsets,cytokine levels,bone marrow nucleated cell count and deoxyribonucleic acid(DNA)content were measured.Results:After modeling,the morphological structure of the spleen and thymus tissues in mice was disordered;white blood cell count,CD3+,CD4+and CD8+lymphocyte percentages,immunoglobulin contents,and the number of nucleated cells and DNA content in bone marrow were significantly reduced(P<0.05);cytokine levels were significantly increased(P<0.05).Compared with the model group,the above indicators in the chickpea peptide intervention groups were improved in different degrees,with the most significant effect being observed at the middle and high doses(P<0.05).Conclusion:Chickpea peptide can enhance the immune function of immunocompromised mice induced by cyclophosphamide.