Candidate genes associated with cold-coagulation or heataccumulation blood stasis syndrome in hypertension

Background:The goal of this study was to predict candidate genes by analyzing the differentially expressed genes of cold-coagulation or heat-accumulation blood stasis syndrome in hypertension by transcriptomes sequencing in human vascular endothelial cells models.Methods:Serum of patients with hypertension were collected to incubate with normal human umbilical vein endothelial cells to establish injured endothelial cell models of cold-coagulation blood stasis syndrome,heat-accumulation blood stasis syndrome and non-blood stasis syndrome.The differentially expressed genes of cold-coagulation blood stasis syndrome or heat-accumulation blood stasis syndrome were screened compared with non-blood stasis syndrome.Gene Ontology,pathway enrichment analyses and PPI network analyses were conducted to get the key genes of cold-coagulation blood stasis syndrome or heataccumulation blood stasis syndrome.Results:The results showed that compared with non-blood stasis syndrome,there were 368 differentially expressed genes in cold-coagulation blood stasis syndrome(275 up-regulated,93 down-regulated),and 271 differentially expressed genes in heat-accumulation blood stasis syndrome(202 upregulated,69 down-regulated).According to the bioinformatics analyses,5 differentially expressed genes were selected as the candidate genes for cold-coagulation blood stasis syndrome(TRIB3,HERPUD1,ERN1,PMAIP1 and XBP1)and 10 differentially expressed genes were selected as the candidate genes for heat-accumulation blood stasis syndrome(PTGS2,SLC3A2,VEGFA,SLC7A5,SLC1A4,SLC7A1,SLC38A1,SLC43A2,HMOX1 and ICAM1).Conclusion:In this study,we predicted the potential key genes associated with cold-coagulation blood stasis syndrome or heat-accumulation blood stasis syndrome in hypertension by transcriptomes sequencing and bioinformatics analyses.It provide an informative basis for studying the role of genes in blood stasis syndrome,and we hope it will be valuable to study blood stasis syndrome in future studies.