长链非编码RNA COL11A1-208在口腔鳞状细胞癌中的表达及临床意义

Expression of long noncoding RNA COL11A1-208 in oral squamous cell carcinoma and its clinical significance

目的·研究长链非编码RNA(long noncoding RNA,lncRNA)COL11A1-208在口腔鳞状细胞癌(简称口腔鳞癌)中的表达水平,并评价其在患者预后预测和诊断中的潜在价值。方法·采用lncRNA基因表达谱芯片检测6对口腔鳞癌组织及对应癌旁正常组织中差异表达的lncRNA;采用实时荧光定量PCR(quantitative real-time PCR,qPCR)检测74对口腔鳞癌组织及癌旁正常组织中COL11A1-208的表达,并比较不同临床特征患者COL11A1-208表达水平的差异。采用受试者工作特征(receiver operating characteristic,ROC)曲线评价癌组织COL11A1-208水平在口腔鳞癌诊断中的潜能。Kaplan-Meier生存分析评估COL11A1-208表达水平对口腔鳞癌患者3年总体生存率的影响。结果·qPCR检测结果显示,COL11A1-208在74例口腔鳞癌组织中的表达显著高于癌旁正常组织(P=0.000),与lncRNA基因芯片的结果一致。结合患者临床特征分析发现,TNM分期较晚(Ⅲ~Ⅳ)的口腔鳞癌组织的COL11A1-208表达水平显著高于分期较早(Ⅰ~Ⅱ)的癌组织(P=0.001);组织COL11A1-208水平用于诊断口腔鳞癌的ROC曲线下面积为0.702(95%CI 0.617~0.788,P=0.000),灵敏度和特异度分别为87.8%和54.1%;高表达COL11A1-208患者3年生存率显著低于低表达者(P=0.002)。结论·COL11A1-208在口腔鳞癌组织内高表达,在TNM分期较晚的癌组织表达水平更高;COL11A1-208可能在口腔鳞癌预后预测及诊断中有潜在应用价值。

Objective·To investigate the expression of long noncoding RNA(lncRNA)COL11A1-208 in oral squamous cell carcinoma(OSCC),and evaluate its potential value in prognosis prediction and diagnosis of OSCC.Methods·The differentially expressed lncRNAs from six pairs of OSCC tissues and the para-carcinoma tissues were detected by lncRNA microarray.Quantitative real-time PCR(qPCR)was used to detect the expression of COL11A1-208 in seventy-four pairs of OSCC tissues and para-carcinoma tissues.The difference of COL11A1-208 expression among the patients with different clinical features were analyzed.The potential effectiveness of COL11A1-208 level in the diagnosis of OSCC was evaluated by the receiver operating characteristic(ROC)curve.Kaplan-Meier survival analysis was used to analyze the effects of COL11A1-208 on the 3-year overall survival rate of OSCC patients.Results·Compared with the para-carcinoma tissues,COL11A1-208 was highly expressed in OSCC tissues(P=0.000),which was consistent with the results of lncRNA microarray.The expression of COL11A1-208 in the patients with advanced TNM stage(Ⅲ-Ⅳ)was significantly higher than that with TNM stageⅠ-Ⅱ(P=0.001).The area under ROC curve of COL11A1-208 as a biomarker for the diagnosis of OSCC was 0.702(95%CI 0.617-0.788,P=0.000),and the sensitivity and specificity were 87.8%and 54.1%,respectively.The 3-year overall survival rate of the patients with low expression of COL11A1-208 was significantly longer than that of the patients with high expression(P=0.002).Conclusion·The expression of COL11A1-208 is up-regulated in OSCC tissues,which is even higher in the patients with advanced TNM stage.COL11A1-208 might have potential value in prognostic prediction and diagnosis of OSCC.