HPV18在不同病理类型宫颈癌中miRNA表达差异研究

Study on the miRNA expression differences in patients with HPV 18 and different pathological types of cervical cancer

目的探究人类乳头瘤病毒18(human papillomavirus 18,HPV18)在不同病理类型宫颈癌中微小核糖核酸(miRNA)表达差异。方法选取30例HPV18阳性宫颈腺癌患者为A组,30例HPV18阳性宫颈鳞癌患者为B组,15例宫颈正常者为C组。验证A组与B组的病灶组织差异表达miRNA,比较三组的差异表达miRNA及靶基因的表达情况,并比较A组中不同年龄、分期、分化程度及转移情况者的表达情况。结果A组与B组的差异表达miRNA为miR-34、miR-19,A组的miR-19及存活素(survivin)表达显著高于B组,miR-34及蛋白酪氨酸磷酸酶基因(phosphatase and tensin homolog deleted on chromosome ten,PTEN)表达显著低于B组,且A组与B组miR-19和survivin高于C组,miR-34和PTEN低于C组,差异有统计学意义(P<0.05),A组中不同年龄者的上述指标表达比较,差异无统计学意义(P>0.05),不同分期、分化程度及转移情况者的上述指标表达比较,差异有统计学意义(P<0.05)。结论miR-19、miR-34、survivin及PTEN在HPV18阳性在不同病理类型宫颈癌中的表达差异显著,且不同分期、分化程度及转移情况者的表达情况差异明显。

Objective To investigate and analyze the micro ribonucleic acid(miRNA)expression differences in patients with human papillomavirus 18(HPV 18)and different pathological types of cervical cancer.Methods Thirty patients with HPV18 positive cervical adenocarcinoma were chosen as the group A,30 patients with HPV18 positive cervical squamous carcinoma at the same time were chosen as the group B,15 persons with normal cervix at the same time were chosen as the group C.Then the tissue differentially expressed miRNA of group A and group B were tested,and the expression situation of differentially expressed miRNA and target gene of three groups were compared,the expression situation of group A with different ages,stages,differentiation degree and transfer situation were compared too.Results The differentially expressed miRNA of group A and group B were miR-34 and miR-19.The miR-19 and survivin of group A were significantly higher than those of group B.The miR-34 and phosphatase and tensin homolog deleted on chromosome ten(PTEN)were significantly lower than those of group B.The expression of group A and group B were significantly different from group C,there were statistically significant differences(P<0.05).The expression situation of group A with different ages were compared,there were no statistically significant differences(P>0.05).The expression situation of group A with different stages,differentiation degree and transfer situation were compared,there were statistically significant differences(P<0.05).Conclusion The expression differences of miR-19,miR-34,survivin and PTEN in patients with HPV 18 and different pathological types of cervical cancer are significant,and the expression differences of patients with different stages,differentiation degree and transfer situation are obvious.