酸处理对马铃薯块茎形成相关基因表达的影响

Effect of Acid Treatment on Expression of Genes Related to Tuber Formation of Potato

为了探讨块茎形成相关基因表达量的变化,利用pH值4的磷酸溶液对植株根进行了4 d的胁迫处理,之后收集匍匐茎茎尖形成弯钩之前(Ⅰ)、匍匐茎弯钩时期(Ⅱ)、匍匐茎刚膨大时期(Ⅲ)、初具块茎形态特征时期(Ⅳ)的匍匐茎作为试验材料。出苗后生长45 d的植株形成的匍匐茎中Ⅱ时期匍匐茎所占比例最高。生长45 d时酸处理的植株所形成的块茎数量最多,其结薯数量比生长55,60 d酸处理植株的结薯数量显著高53.2%,74.5%。匍匐茎Ⅰ、Ⅱ、Ⅲ、Ⅳ时期,酸处理植株的StHd3a、StFD、StSUSY4、StHXK、StPIN1、StABA、StSP6A表达量均显著高于对照组;匍匐茎Ⅰ时期和Ⅱ时期,酸处理植株的StGA的表达量比对照显著高49.4%,25.1%,StAGL8的表达量比对照显著高119.7%,140.1%,而StAGPase的表达量比对照显著低60.1%,41.7%,StSSS的表达量比对照组显著低23.2%,21.6%;另外,酸处理植株StCOL的表达量与对照相比无显著性差异。综上,酸处理是通过调控被测基因的表达量或表达模式(除StCOL基因)而影响块茎形成的复杂过程。

The roots of potato plants were treated with phosphoric acid solution(pH 4)for 4 d,then four stages of stolons were collected as samples,which were before stolon tip hook(stageⅠ),stolon tip hook(stageⅡ),swelling tips(stageⅢ)and initial tuber formation(stageⅣ).The samples were used to determine the expression levels of genes related to tuber formation.The 45-day-old plants treated with acid solution formed the maximum stolon at stageⅡ,and produced the largest quantity of tubers which were 53.2%and 74.5%higher than that of the 55 and 60-day-old plants.The expressions levels of StHd3a,StFD,StSUSY4,StHXK,StPIN1,StABA and StSP6A of acid treatment group were obviously higher than those of control group in all stages.Moreover,at the stolon development in acid treatment group of stageⅠand stageⅡ,compared with the control group,the StGA expressions were significantly increased by 49.4%and 25.1%,the StAGL8 expressions were significantly increased by 119.7%and 140.1%,while the StAGPase expressions were significantly reduced by 60.1%and 41.7%,and the StSSS expressions were significantly reduced by 23.2%and 21.6%.However,the StCOL expressions in acid treated plants were not significantly different from that of the control grup.In conclusion,the acid treatment could affect the complex process of potato tuber formation through the regulation of the above tested genes(except StCOL)expression levels or patterns during the tuberization.