lncRNA HOTAIR 和H19 SNPs与胃癌及EB病毒相关胃癌遗传易感性的关系

Relationships between lncRNA HOTAIR/H19 SNPs and genetic suscepti⁃bility to gastric carcinoma/EBV-related gastric carcinoma

目的:探讨长链非编码RNA(lncRNA)H19及HOTAIR基因多态性与胃癌和EB病毒相关胃癌(EBVaGC)易感性的关系。方法:研究对象选择EB病毒阴性胃癌(EBVnGC)组织65例、EBVaGC组织50例及115例健康人外周血标本,并从中提取其DNA备用。选择H19 rs3024270和rs3741219,以及HOTAIR rs4759314和rs874945共4个基因多态性位点,利用Taq-Man MGB等位基因分型试剂盒对各单核苷酸多态性(SNP)位点基因进行分型,统计分析实验结果。结果:H19及HOTAIR的SNPs在全部标本中均符合Hardy-Weinberg平衡。(1)H19 rs3024270位点的SNP在胃癌组与对照组中的差异有统计学意义(P<0.05);其中G等位基因为保护基因,个体携带G等位基因可显著降低罹患胃癌的风险(P<0.01);(2)HOTAIR rs4759314位点在胃癌组与对照组中基因型和等位基因分布频率均有显著差异(P<0.05);基因型为GG的人群,胃癌的发病风险显著增加(P<0.05);且胃癌的风险基因可能是G等位基因,携带G等位基因人群罹患胃癌的风险显著增加(P<0.05);(3)H19 rs3741219及HOTAIR rs874945位点的各基因型及等位基因频率在两组间无显著差异(P>0.05);(4)HOTAIR rs4759314位点G等位基因在EBVaGC组的分布高于EBVnGC组,而EBVaGC和EBVnGC组间其他SNPs的分布差异均无统计学意义(P>0.05)。结论:H19 rs3024270和HOTAIR rs4759314位点的SNPs与胃癌的发病风险有关,但与EBVaGC的发病风险之间未观察到明确的关联性。

AIM:To investigate the potential associations between the single nucleotide polymorphisms(SNPs)of long noncoding RNA(lncRNA)H19/HOTAIR and the susceptibility to gastric carcinoma,especially to Epstein-Barr virus(EBV)-associated gastric carcinoma(EBVaGC).METHODS:Peripheral blood samples from 65 cases of EBV-negative gastric carcinoma(EBVnGC),50 cases of EBVaGC and 115 cases of healthy people were collected.A total of 4 TagSNPs,H19 rs3024270 and rs3741219,as well as HOTAIR rs4759314 and rs874945,were selected.The Taq-Man MGB allele typing kit was used to detect the genotype of each SNP locus,and the experimental results were statistical⁃ly analyzed.RESULTS:(1)There were significant differences of both genotypic and allelic frequencies at H19 rs3024270 locus between gastric carcinoma group and control group(P<0.05).Individuals carrying the G allele at H19 rs3024270 locus had significantly low risk of gastric carcinoma(P<0.01),indicating that the G allele was protective.(2)People with the GG genotype at HOTAIR rs4759314 locus had significantly high risk of gastric carcinoma(P<0.05).Car⁃rying the G allele increased the risk of gastric carcinoma,which indicated that the risk gene for gastric carcinoma might be the G allele.(3)No significant difference of the genotypic and allelic frequencies at H19 rs3741219 and HOTAIR rs874945 loci between gastric carcinoma group and control group was observed(P>0.05).(4)The G allele frequency at HOTAIR rs4759314 locus in EBVaGC group was significantly higher than that in EBVnGC group.However,no difference of the other 3 SNPs was found between EBVaGC group and EBVnGC group(P>0.05).CONCLUSION:The SNPs at H19 rs3024270 and HOTAIR rs4759314 loci are related to the risk of gastric carcinoma,but not significantly related to the risk of EBVaGC.