单宁酸-玉米黄粉蛋白复合物抗氧化性研究

Antioxidant Activity of Tanning Acid-Corn Gluten Meal Complex

将玉米黄粉蛋白(Corn Yellow Protein,CYP)与0.04%的单宁酸(Tannic Acid,TA)复合,利用碱性蛋白酶制得单宁酸-玉米黄粉蛋白(Tannic Acid-Corn Yellow Protein,TA-CYP)多肽,对其抗氧化活性进行测定,并做性质表征。结果表明:单宁酸-玉米黄粉蛋白复合物比CYP的可溶性蛋白含量提高78%;CYP与TA-CYP的DPPH自由基清除率分别为51.37%、63.56%(P<0.05),抗氧化性显著提高;最优酶解工艺为:酶浓度2%、底物浓度100 mg/mL、温度40℃、p H8.0、酶解3 h,在此条件下制备TA-CYP复合物抗氧化活性肽,傅里叶红外光谱和扫描电子显微镜结构均有明显变化,DPPH自由基清除能力、·OH自由基清除能力、O2-·自由基清除能力、还原力和Fe2+螯合能力均保持在较高的状态。

In this paper,corn yellow protein(CYP)was combined with 0.04%tannic acid(TA)and the tannic acid-corn yellow protein(TA-CYP)polypeptide was prepared by alkaline protease.The antioxidant activity as well as physicochemical property was determined.The content of protein of TA-CYP was 78%higher than that of CYP.The DPPH radical scavenging ability of CYP and TA-CYP was 51.37%and 63.56%(P<0.05)respectively,which indicated that the antioxidant activity of CYP was enhanced markedly.TA-CYP polypeptides with antioxidant activity were prepared under the optimal enzymatic hydrolysis processes:enzyme concentration 2%,substrate concentration 100 mg/mL,temperature 40℃,pH 8.0,enzymatic hydrolyzed for 3 h.The TA-CYP polypeptides prepared through these conditions showed obvious changes in structure of Fourier transform infrared spectroscopy and scanning electron microscope,and the DPPH radical scavenging ability,·OH radical scavenging ability,O2-·radical scavenging ability,reducing power and Fe2+chelating ability exhibited a high level.