活性炭法制备高F值栉孔扇贝酶解液的工艺条件

Process conditions of enzymatic hydrolysis of scallop Chlamys farreri with high Fischer ratio by activated carbon method

采用OD(220)/OD(280)、支链氨基酸保留率和芳香族氨基酸去除率3个指标,从5种活性炭中筛选脱除栉孔扇贝酶解液中芳香族氨基酸效果最优的材料。通过单因素和正交实验研究温度、pH、吸附时间、固液比对筛选得到的活性炭吸附效果的影响,并优化活性炭吸附工艺条件,制备出符合高F值要求的栉孔扇贝酶解液。结果表明,200目粉末活性炭A吸附效果最好,最佳吸附条件为温度35℃、pH 6、吸附时间2 h、固液比1∶15(g∶mL);经活性炭吸附后,酶解液中的支链氨基酸保留率达到73.869%,芳香族氨基酸脱除率达到93.120%,酶解液F值达到34.73。该活性炭吸附脱芳技术可以用于制备栉孔扇贝高F值寡肽溶液。

The materials with the best removing effect of aromatic amino acids from the enzymolysis solution of scallop(Chlamys farreri)were selected from five kinds of activated carbon using OD 220/OD 280,branched-chain amino acid retention rate and aromatic amino acid removal rate as indexes.Through single factor and orthogonal experiments,the effects of temperature,pH,adsorption time,solid-liquid ratio on the adsorption effect were studied,and the activated carbon adsorption process conditions were optimized to prepare the enzymolysis solution of scallop with high F value.The results showed that the adsorption effect of 200 mesh powdered activated carbon A was the best.The optimal adsorption conditions were 35℃,pH 6,adsorption time 2 h and solid-liquid ratio 1∶15(g∶mL).After adsorption by activated carbon,the retention rate of branched amino acids in enzymatic hydrolysate reached 73.869%,the removal rate of aromatic amino acids reached 93.120%,and the Fischer ratio of enzymatic hydrolysate reached 34.73.The activated carbon adsorption and desorption aromatic amino technology can be used to prepare high Fischer ratio oligopeptide solution of scallop.