新疆桃果内生细菌群落组成分析

Community composition analysis of endophytic bacteria in Xinjiang peaches

为解析桃果采后内生细菌群落结构组成及多样性,挖掘其中潜在的益生微生物资源。该文利用高通量测序技术对新疆不同品种的桃果内生细菌进行扩增与分析。结果表明,3类桃果内生细菌共包括106个操作分类单元(operational taxonomic units,OTU),涉及9个门,59个属,包含73个相同OTUs。进一步分析发现,水蜜桃中主要菌群为泛菌属和芽孢杆菌属,其中泛菌属菌群数占到总数的1/3以上;蟠桃中主要菌群为假单胞菌属和未分类菌属,约占总体的19.83%、16.57%。油桃中主要菌群为芽孢杆菌属和未分类属,约占总体的15.63%、15.15%;不动杆菌属(Acinetobacter)、拟杆菌属(Bacteroides)为油桃、水蜜桃和蟠桃的共同优势菌群,分别约占总体的7.41%、10.58%、14.49%和8.65%、4.30%、8.91%。同时,研究发现双歧杆菌属(Bifidobacterium)、乳酸杆菌属(Lactobacillus)、阿卡曼氏属(Akkermansia)等与人体健康相关的菌群大量存在于桃果中。此外,桃果中存在大量未知分类菌群。该文为进一步分析桃果内生细菌群落与人体健康潜在的相互关系,深度挖掘与利用内生细菌资源研究提供了研究基础。

In order to analyze the composition and diversity of endophytic bacteria communities and explore the potential probiotics resources in peaches,high-throughput sequencing was performed to amplify and analyze the endophytic bacteria communities from different Xinjiang peaches.The results showed that 106 operational taxonomic units(OTUs)including 59 genera in 9 phyla were obtained,in which 73 OTUs co-existed in these three kinds of peaches.Pantoea and Bacillus were the major genera in honey peach,of which Pantoea accounted for more than 1/3 of the total.The main genera in peento were Pseudomonas and unclassified bacteria,which accounted for 19.83%and 16.57%,respectively.While Bacillus and unclassified bacteria were found densely in the nectarine accounting for 15.63%and 15.15%,respectively.Additionally,Acinetobacter and Bacteroides were the dominant bacteria in these three kinds of peaches,accounting for 7.41%,10.58%,14.49%and 8.65%,4.30%,8.91%of the total respectively.Furthermore,many endophytic bacteria in Bifidobacterium,Lactobacillus and Akkermansia related to human health were observed,and many unclassified endophytic bacteria were detected in peaches.It provides a scientific basis to analysis the potential relationship between endophytic bacteria and human health,and to utilize the resources of endophytic bacteria in peaches.