三七提取物的抗衰老功效性研究

Study on the anti-aging efficacy of Sanqi extract

建立了衰老动物模型并对化妆品原料三七提取物的抗衰老功效进行评价。采用皮下注射D-半乳糖连续45 d得到衰老小鼠模型,在无毛区连续涂抹样品50 d。采用多功能皮肤分析仪对小鼠皮肤的肤色、色素及毛孔等指标进行测定。通过免疫组织化学染色对I型胶原蛋白(Col-I)、III型胶原蛋白(Col-III)与波形蛋白(VIM)进行观察。对皮肤组织匀浆进行总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)含量测定以评估样品处理后抗氧化能力的变化。结果表明,通过免疫组织化学染色可知4%三七软膏组可明显提高Col-I、Col-III与VIM的含量;三七软膏对T-SOD和GSH-Px的上调作用具有较好的剂量依赖效果。其中4%三七软膏组与空白基质组相比,T-SOD活力值增加了36.62%,CAT活力值增加了20.39%,GSH-Px活力值增加了68.77%。

An aging animal model was established and the anti-aging effects of Sanqi extract as a cosmetic ingredient were evaluated.D-galactose was injected subcutaneously for 45 days to obtain an aging mouse model.The samples were continuously applied to the hairless area for 50 days.Multifunctional skin analyzer was used to measure the skin color,pigment,and pores of the mouse skin.Collagen I(Col-I),Collagen III(Col-III)and vimentin(VIM)were evaluated by immunohistochemical staining.Total superoxide dismutase(T-SOD),catalase(CAT),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in skin tissue homogenates were measured to evaluate the changes in antioxidant capacity after sample treatment.The results showed that the ointment containing 4%Sanqi extract could significantly increase the content of Col-I,Col-III and VIM by immunohistochemical staining.Sanqi ointment had a good dosage-dependent effect on the up-regulation of T-SOD and GSH-Px.Compared with the blank matrix group,the T-SOD activity was increased by 36.62%,the CAT activity was increased by 20.39%,and the GSH-Px activity was increased by 68.77%after treatment with the ointment containing 4%Sanqi extract.