滇黄精提取物成分与活性测定及细胞毒性评价

Determination of components and activity of extractions of Polygonatum kingianum and evaluation of its cytotoxicity

制备滇黄精提取物,测定多糖、多酚、皂苷和黄酮类成分含量,以及抗氧化能力和酪氨酸酶抑制活性,并采用细胞试验研究提取物安全性,评价黄精提取物在日化产品中的应用潜力。结果表明:干制、蒸制和酒制滇黄精提取物总多糖、多酚、皂苷和黄酮含量分别为39.68%~56.33%,0.31%~4.11%,4.10%~8.54%,0.14%~0.47%;三种提取物都具有一定的还原力、较高的羟自由基和DPPH自由基清除率以及酪氨酸酶抑制活性,并且同一浓度下酒制黄精提取物活性显著高于另外两种提取物(P<0.05)。角质形成细胞和成纤维细胞活性测定发现,0.2%含量内的酒制黄精提取物无细胞毒性。综上,黄精提取物尤其是酒制滇黄精提取物含有多糖、皂苷等活性成分,具有抗氧化与酪氨酸酶抑制活性,且无细胞毒性,在日化产品中具有重要的应用价值。

Extractions of Polygonatum kingianum were prepared,and the contents of polysaccharide,polyphenols,saponins,flavonoids,as well as the antioxidant activity and tyrosinase inhibitory activity were determined.The safety of the extraction was studied by cell test,and its application potential in daily chemical products was evaluated.The results show that the contents of total polysaccharides,polyphenols,saponins,flavonoids of dried,steamed and alcoholic extractions of P.kingianum were 39.68%-56.33%,0.31%-4.11%,4.10%-8.54%,0.14%-0.47%,respectively.The three kinds of extractions show a certain reducing power,high scavenging rate of hydroxyl radical and DPPH free radical,and tyrosinase inhibitory activity.With the same concentration,the activity of the alcoholic extraction of P.kingianum is significantly higher than the other two extractions(P<0.05).The activities of keratinocytes and fibroblast cells reveal that 0.2%of alcoholic extraction of P.kingianum is noncytotoxic.Together,the extraction of P.kingianum,especially the alcoholic extraction,are rich in polysaccharides,saponins and other active compounds,and show antioxidant and tyrosinase inhibitory activities without cytotoxicity.It has an important application value in daily chemical products.