Wentilactone A的遗传毒性评价

Genotoxicity evaluation of Wentilactone A

目的检测Wentilactone A的遗传毒性。方法应用经典遗传毒性检测组合(Ames试验、体外培养CHO细胞染色体畸变试验和小鼠骨髓微核试验)检测Wentilactone A的遗传毒性。结果Ames试验结果提示,Wentilactone A在每皿5000、500、50、5、0.5μg 5个剂量下,在加和不加代谢活化系统(S9)时,对鼠伤寒沙门菌均无致突变性。CHO细胞染色体畸变试验结果提示,在终浓度23.74、47.48、94.96μg/ml 3个剂量组,在加和不加S9中,于作用4 h和24 h的条件下培养的CHO细胞,均未诱发染色体畸变。小鼠骨髓微核试验在100、200、400 mg/kg 3个剂量下作用24 h以及400 mg/kg剂量下作用48 h对骨髓细胞的微核诱发率,与溶剂对照组比较均无显著差异(P>0.05)。结论Wentilactone A对鼠伤寒沙门菌无致突变性,对CHO细胞的染色体无致畸变作用,对ICR小鼠无诱发骨髓细胞微核的效应。上述结果提示Wentilactone A不具有遗传毒性和潜在致癌性。

Objective To evaluate the genetic toxicity of Wentilactone A.Methods The classical genotoxicity test combination(Ames test,in vitro CHO cell chromosome aberration test and mouse bone marrow micronucleus test)was used to detect the genotoxicity of Wentilactone A.Results Ames test suggested that Wentilactone A was not mutagenic against Salmonella typhimurium with or without the metabolic activation system(S9)at five doses of 5000,500,50,5,and 0.5μg/dish.CHO cell chromosome aberration test suggested that the CHO cells cultured in 4 h and 24 h did not induce chromosomal aberrations in three dose groups at the final concentration of 23.74,47.48,94.96μg/ml,with and without S9.The mouse bone marrow micronucleus test showed no significant difference in the bone marrow micronucleus induction rate of cells at three doses of 100,200,and 400 mg/kg treated for 24 h and at dose of 400 mg/kg treated for 48 h compared with the solvent control group(P>0.05).Conclusion These results indicated that Wentilactone A did not exhibit genetic toxicity based on the Ames test,CHO chromosomal aberration test and micronucleus assay.It was suggested that Wentilactone A had no genetic toxicity and potential carcinogenicity.