响应面优化都匀毛尖茶多酚的提取工艺

Response Surface Methodology for Optimization Extraction of Tea Polyphenols from Duyun Maojian

本实验以都匀毛尖为原料,采用超声波辅助提取都匀毛尖茶多酚,利用分光光度法测定含量,选择茶水比、提取时间、提取温度为影响因素,应用Box-Behnken中心组合进行三因素三水平设计,以茶多酚提取率为响应值,进行响应面分析。结果表明:茶多酚的最优佳提取工艺条件为茶水比1:50(g:mL)、提取时间4 min、提取温度100℃。在此条件下茶多酚的提取率为30.15%。响应面结合超声波辅助提取都匀毛尖茶多酚具有能耗低、耗时短、提取率高等特点。

Utrasonic-assisted extraction technology was used to extract tea polyphenols from Duyun Maojian.Spectrophotometry was adopted to measure the content and three independent factors of ratio of water to raw material,extraction time and extraction temperature were selected as affecting factors during extraction.The experiments were arranged according to Box-Behnken central composite experiment design.Response surface analysis method was applied to determine the effect of central composite experiment design.The experimental results showed that the best extraction conditions were as follows:ratio of water to raw material was 1 g:50 mL,extracted time was 4 min,extracted temperature was 100℃.The yield of tea polyphenols reached 30.15%under these conditions.The response surface combined with ultrasonic-assisted extraction of tea polyphenols had the characteristics of low energy consumption,short time consumption and high extraction rate.