烟草根腐病发生的土壤微生物功能多样性分析

An Analysis of Function Diversity of Soil Microbes in Relation to the Outbreak of Tobacco Root Rot

烟草根腐病是河南省南阳市烟区的一种重要病害,随土壤连作时间的增加,根腐病的发生日益严重且发病时间提前,成为制约南阳烟草行业持续健康发展的主要因子.本研究采集了南阳地区不同连作年限烟田中根腐病发病和无病土壤,采用Biolog-ECO微平板技术对土壤微生物功能多样性进行了评估,初步明确了随烟草连作年限的增加,根腐病发生程度加重,土壤微生物对碳源的代谢能力呈增强的趋势,而发病土壤微生物功能多样性和均匀度均明显低于无病土壤,发病土壤微生物代谢土壤中4-羟基苯甲酸能力不足可能是引起根腐病发生的重要因子.本研究结果为破解南阳烟区根腐病发生的微生态机制提供了理论参考.

Root rot of tobacco is an important disease in tobacco-growing areas in Nanyang.With the increase in soil continuous cropping time,the occurrence of root rot is becomingmore and more serious and the onset time is advanced in time,which has become the main restricting factor for the sustainable and healthy development of tobacco industry in Nanyang.In order to provide a theoretical reference for understanding the microbial mechanism of root rot in Nanyang tobacco-growing areas a study was made,in which soil samples with or without root rot disease were collected from Nanyang with different years of tobacco continuous cropping,and the Biolog-ECO micro-tablet technology was employed to evaluate the soil microbial functional diversity.It was tentatively concluded that with increasing years of continuous tobaccocropping,occurrence of root rot becomes more severe,and soil microbial metabolic ability of carbon sources tends to be enhanced,that soil microbial functional diversity and evenness in the diseased soil are significantly lower than in the disease-free soil,and that the deficiency in the ability of soil microbes in a diseased soil to metabolize 4-hydroxybenzoic acid may be the most important factor for root rot occurrence.