Bioactive Constituents of the Seed Kernels from Vernicia fordii

A new compound,(2′R)-(5-oxo-2,5-dihydrofuran-2-yl)methyl octanoate(1),together with 9 known ones(2-10)were isolated from the bioactive extract of the seed kernels from Vernicia fordii.Their structures were determined by spectroscopic analyses,including Nuclear Magnetic Resonance(NMR)and High Resolution Electrospray Ionization Mass Spectroscopy(HRESI-MS).Their cytotoxicities against three human cancer cell lines(A549,HepG2 and Caco2 cells)and anti-neuroinflammatory effects were evaluated.Cytotoxicity assay revealed that compound 9 could inhibit the growth of HepG2 cells,while compound 6 had a moderate inhibitory effect against A549 cells.Further anti-neuroinflammatory activity assay showed that compound 10 exhibited moderate inhibitory effect on the release of NO in LPS-stimulated BV2 microglia cells.