JAK1在喉鳞状细胞癌中的表达及临床意义

Expression and clinical significance of JAK1 in laryngeal squamous cell carcinoma

目的检测酪氨酸激酶1(JAK1)在喉鳞状细胞癌组织中的表达,并分析其与临床病理特征的相关性,探讨JAK1的临床意义。方法应用RT-qPCR检测mRNA表达和Western Blot检测蛋白表达,同时采用免疫组织化学技术检测其在喉鳞癌组织及癌旁组织中的表达,根据免疫组化评分分析其与临床病理特征的关系。结果与癌旁组织相比,喉鳞癌组织JAK1mRNA明显增高,P<0.01;与癌旁组织相比,喉鳞癌组织JAK1蛋白表达明显增高,P<0.01;JAK1表达与肿瘤分化程度、临床分期、淋巴结转移等因素密切相关,P<0.05。结论JAK1在喉癌组织中表达升高,在喉癌发生、发展及转移过程中起重要作用。

Objective To detect the expression of Tyrosine kinase1(JAK1)in laryngeal squamous cell carcinoma and analyze its correlation with clinicopathological features,in order to explore the clinical significance of JAK1.Methods The expression of mRNA and protein of JAK1 in laryngeal squamous cell carcinoma and adjacent tissues was detected by Real-time quantitative polymerase chain reaction(RT-qPCR)and Western-blot(Western blotting),the expression of mRNA and protein in laryngeal squamous cell carcinoma and paracancerous tissues was detected by immunohistochemical method,and the relationship between the expression and clinicopathological features was analyzed according to the method of immunohistochemical score.Results The expression of JAK1 in mRNA in laryngeal squamous cell carcinoma was significantly higher than that in paracancerous tissues,P<0.05;the expression of JAK1 in protein in laryngeal squamous cell carcinoma was significantly higher than that in paracancerous tissues,P<0.05;the results of immunohistochemical showed the expression of JAK1 in laryngeal squamous cell carcinoma was related to the degree of differentiation,clinicopathological stage and lymph node metastasis,P<0.05.Conclusion The increased expression of JAK1in laryngeal squamous cell carcinoma plays an important role in the occurrence,development and metastasis of laryngeal carcinoma.