NAFLD对大鼠葡萄糖、胰岛素代谢和肝糖原合成的影响

Effects of NAFLD act on glucose,insulin metabolism and liver glycogen synthesis in rats

目的:探讨非酒精性脂肪肝(NAFLD)对大鼠葡萄糖、胰岛素代谢和肝糖原合成的影响。方法:通过高脂饮食建立NAFLD大鼠模型,穿刺肝静脉取血,检测肝脏葡萄糖、胰岛素代谢能力和糖原合成能力;蛋白质印迹法和RT-PCR检测糖原合成和糖异生相关酶基因及蛋白的表达。结果:与对照组相比,NAFLD模型组大鼠的葡萄糖、胰岛素代谢速度及肝脏糖原储备速度均显著降低,葡萄糖耐量和胰岛素耐受水平均下降,肝脏糖原合成相关酶基因和蛋白的表达水平均显著降低,肝脏糖原合成相关酶的磷酸化水平均显著降低,而糖异生相关基因的表达水平则显著升高,差异均有统计学意义(均P<0.05)。结论:NAFLD大鼠肝脏葡萄糖、胰岛素代谢能力下降,肝糖原合成减少,导致高血糖和高胰岛素血症,增加胰岛素抵抗和患2型糖尿病的危险。

Objective:To investigate the effects of non-alcoholic fatty liver disease(NAFLD)on glucose,insulin metabolism and hepatic glycogen synthesis in rats.Methods:NAFLD rat model was established by high-fat diet,blood was collected from hepatic vein,and glucose metabolism,insulin metabolism and glycogen synthesis were detected respectively.Glycogen synthesis and expression of gluconeogenesis related enzyme genes and proteins were detected by Western blotting and RT-PCR.Results:Compared with the control group,the glucose metabolism,insulin metabolic rate and liver glycogen reserve rate in NAFLD model group were significantly decreased,and the liver glycogen synthesis-related enzymes phosphorylation level was significantly decreased,however,the expression level of gluconeogenesis related genes increased significantly,the difference were all significant(all P<0.05).Conclusion:In NAFLD rats,the liver's ability to regulate glucose and insulin metabolism is decreased,liver glycogen synthesis is decreased,which results in hyperglycemia and hyperinsulinemia,and increases insulin resistance and the risk of type 2 diabetes.