LncRNA TUG1靶向miR-138-5p调控胰腺癌细胞增殖、迁移和侵袭

LncRNA TUG1 regulates proliferation,migration and invasion of pancreatic cancer cells by targeting miR-138-5p

目的:探讨长链非编码RNA牛磺酸上调基因1(TUG1)和微小RNA 138-5p(miR-138-5p)在胰腺癌组织中的表达及其对胰腺癌细胞增殖、迁移和侵袭的影响。方法:应用RT-qPCR法检测胰腺癌组织及其细胞系中TUG1和miR-138-5p表达水平。通过小干扰RNA下调PANC-1细胞中TUG1水平或转染miR-138-5p mimics至PANC-1细胞,MTT、Transwell法检测细胞活力、迁移和侵袭。starBase v2.0在线预测和双荧光素酶报告基因实验验证LncRNA TUG1和miR-138-5p的靶向关系。共转染TUG1-siRNA和miR-138-5p mimics至PANC-1细胞,MTT、Transwell法检测细胞活力、迁移和侵袭。结果:在胰腺癌组织及其细胞系中,TUG1表达上调(P<0.05)而miR-138-5p表达下调(P<0.05)。在PANC-1细胞中敲减TUG1或过表达miR-138-5p,细胞活力、迁移和侵袭能力下降(P<0.05)。starBase v2.0在线预测和双荧光素酶报告基因实验结果表明,TUG1可靶向调控miR-138-5p表达。MTT、Transwell实验结果显示,降低miR-138-5p的表达可逆转TUG1-siRNA对PANC-1细胞增殖、迁移和侵袭的抑制作用。结论:敲减TUG1能够通过上调miR-138-5p水平抑制胰腺癌细胞增殖、迁移和侵袭。

Objective:To detect the expression of long-chain non-coding RNA taurine up-regulated gene 1(TUG1)and microRNA 138-5 p(miR-138-5 p)in pancreatic cancer tissues,and investigate their effect on proliferation,migration and invasion of pancreatic cancer cells.Methods:The expression of TUG1 and miR-138-5 p in pancreatic cancer tissues and cell lines were detected by RT-qPCR.Cell viability,migration and invasion of PANC-1 human pancreatic cancer cells after transfection of TUG1 siRNA or miR-138-5 p mimics were detected by MTT and Transwell assay,respectively.The targeting relationship between lncRNA TUG1 and miR-138-5 p was verified by dual-luciferase assay and starBase v2.0 online prediction.Cell viability,migration and invasion of PANC-1 cells after co-transfection of TUG1-siRNA and miR-138-5 p mimics were detected by MTT and Transwell assay,respectively.Results:The expression of TUG1 was increased in pancreatic cancer tissues and cell lines(P<0.05)while the expression of miR-138-5 p was decreased(P<0.05).Cell viability,migration and invasion of PANC-1 cells after transfection of TUG1 siRNA or miR-138-5 p mimics were decreased(P<0.05).The results of starBase v2.0 online prediction and daul-luciferase assay indicted that TUG1 could regulate the expression of miR-138-5 p.And reducing of miR-138-5 p expression could reverse the inhibition of TUG1-siRNA on proliferation,migration and invasion of PANC-1 cells.Conclusion:Knockdown of TUG1 could inhibit the proliferation,migration and invasion of pancreatic cancer cells by targeting miR-138-5 p.