便携式癌细胞荧光显微成像系统的设计与实现

Design and Implementation of a Portable Fluorescence MicroscopicImaging System for Cancer Cells

根据荧光成像在癌症诊断分析中的机理,选择高效率的光学系统,配合高精度的半数字式对焦技术和智能图像采集融合技术,实现高性能便携式癌细胞荧光成像系统。系统选择多波段的LED光源;采用科学级面阵互补金属氧化物半导体(CMOS)探测器,配合散斑抑制二向色镜,降低背景干扰,提高检测灵敏度;采用半数字式对焦技术,实现显微镜高精细的自动对焦;采用FPGA+万兆网的结构,实现图像数据的高速传输。实验表明,基于该系统可制成质量为4 kg、功耗不到20 W的便携式癌细胞荧光成像设备,实现清晰的多谱段癌细胞荧光成像和快速检测。

Based on the mechanism of fluorescence imaging in cancer diagnosis and analysis,a high-performance portable cancer cell fluorescence imaging system is developed with a high-efficiency optical system,using high-precision semi-digital focusing technology and intelligent image acquisition and fusion technology.The system selects multi-band LED light source,uses sci area array CMOS detector and speckle suppression dichroic mirror,which reduces background interference and improve detection sensitivity,adopts semi-digital focusing technology which achieves high-precision autofocus of the microscope;and applies sing FPGA+10 Gigabit network structure so that high-speed transmission of image data is realized.Experiments show that based on this system,a portable fluorescence imaging device for cancer cells with a mass of 4 kg and a power consumption of less than 20 W can be made,which can realize clear multi-spectral fluorescence imaging and rapid detection of cancer cells.