枸杞多糖对高糖环境下HRCEC增殖及VEGF表达的影响

Effects of Lycium Barbarum Polysaccharide on Proliferation and VEGF Expression of HRCEC under High Glucose

目的观察不同浓度的枸杞多糖(lycium barbarum polysaccharide,LBP)对体外高糖环境下培养的人视网膜血管内皮细胞(human retinal capillary endothelial cells,HRCEC)增殖及血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法获取人眼球摘除术后的HRCEC,予以体外培养,选取最佳状态的3~4代细胞;CCK8法检测在不同浓度LBP体外高糖环境下分别作用12、24、36 h后HRCEC的增殖情况;Western blot法检测各组HRCEC的VEGF表达情况。结果同一时间组间比较,高糖组HRCEC活性及VEGF的表达量均高于低糖组(P<0.05);不同浓度LBP实验组HRCEC活性及VEGF表达量均明显低于高糖组(P<0.05),80μg/mL LBP组HRCEC活性及VEGF表达低于20μg/mL LBP实验组与40μg/mL LBP实验组(P<0.05);各实验组在干预36 h时,HRCEC活性及VEGF表达低于干预12 h与24 h(P<0.05)。结论LBP能抑制高糖环境下HRCEC的增殖并下调VEGF的表达,并与LBP浓度和作用时间正相关。

Objective To observe the effects of different concentrations of Lycium barbarum polysaccharide(LBP)on the proliferation of human retinal capillary endothelial cells(HRCEC)and the expression of vascular endothelial growth factor(VEGF)cultured in a high glucose environment in vitro.Methods The HRCEC was obtained after human eyeball enucleation,and cultured in vitro.The best 3 to 4 generation cells were selected;CCK8 method was used to detect the proliferation of HRCEC after 12 h,24 h,36 h under high glucose environment by different concentration of LBP in vitro;Western Blot method was used to detect VEGF expression of HRCEC in each group.Results In comparison between groups at the same time,HRCEC activity and VEGF expression in the high glucose group were higher than those in the low glucose group(P<0.05);HRCEC activity and VEGF expression in the LBP experimental group at different concentrations were significantly lower than those in the high glucose group(P<0.05).HRCEC activity and VEGF expression in 80μg/m LLBP group were lower than 20μg/mL LBP experimental group and 40μg/mL LBP experimental group(P<0.05);HRCEC activity and VEGF expression in each experimental group with intervention for 36 h were lower than 12 h and 24 h(P<0.05).Conclusion LBP can inhibit the proliferation of HRCEC and down-regulate the expression of VEGF in a high glucose environment,and is positively correlated with the concentration of LBP and the duration of action.