A standardized crush tool to produce consistent retinal ganglion cell damage in mice

In the mammalian central ner vous system, neuronal loss induced by injuries or in neurodegenerative diseases is often irreversible(Quigley, 2016;Gan et al., 2018).Following the disease insult, the surviving neurons may continue to lose their functionality because their axons degenerate and fail to maintain proper synaptic connections, and the underlying molecular and cellular mechanisms remain to be investigated(Raff et al., 2002;Bei et al., 2016;Quigley, 2016).