摘要
为明确猕猴桃软腐病病原菌种类及香芹酚对其控制效果,依据传统真菌形态学观察法和rDNA-ITS序列分析技术对发病猕猴桃果实中的致病菌进行病原菌的分离鉴定,并探讨香芹酚对分离得到的猕猴桃软腐病菌的控制效果。结果表明:引起猕猴桃采后果实软腐病的主要致病菌为葡萄座腔菌(Botryosphaeria dothidea)与拟茎点霉菌(Phomopsis sp.),其次为灰葡萄孢菌(Botrytis cinerea)、层出镰刀菌(Fusarium proliferatum)和链格孢菌(Alternaria sp.);香芹酚对8株猕猴桃软腐病病原真菌均表现出良好的抑菌活性,对葡萄座腔菌和拟茎点霉菌的抑制作用尤为显著,半抑制质量浓度(EC50)为8.87μg/L。同时,香芹酚有效控制模拟销售环境下猕猴桃果实软腐病的发生,放置8 d时处理组发病率仅为对照果实的50.17%,且对猕猴桃果实的品质指标影响不显著(P>0.05)。因此,香芹酚在控制猕猴桃果实采后软腐病方面具有潜在的应用价值。
The pathogenic fungi causing kiwifruit soft rots were isolated and identified by examining the morphological and microscopic characteristics,combined with the results of pathogenicity testing and rDNA-ITS(internal transcribed spacer)sequencing.The contril effect of carvacrol on kiwifruits soft rot was studied.The main pathogens causing soft rot of kiwifruit after harvest are Botryosphaeria dothidea and Phomopsis sp.,followed by Botrytis cinerea,Fusarium proliferatum and Alternaria sp..Carvacrol had significant antifungal activity against the eight soft rot pathogens,especially for B.dothidea,with half effective concentration(EC50)of 8.87μg/L.Simultaneously,carvacrol effectively reduced the incidence of kiwifruit soft rot in a simulated sales environment.The incidence of treatment group was only 50.17%of the control fruit when placed for 8 days,and it had no significant effect on the quality of kiwi fruit(P>0.05).Therefore,carvacrol has potential application value in controlling soft rot of kiwifruit after harvest.
作者
左盼盼
付苏
彭丽桃
范刚
杨书珍
李杰
ZUO Panpan;FU Su;PENG Litao;FAN Gang;YANG Shuzhen;LI Jie(College of Food Science and Technology,Huazhong Agricultural University,Wuhan,430070,China)
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2020年第6期15-22,共8页
Journal of Huazhong Agricultural University
基金
国家重点研发计划项目(2019YFD1002300)。
关键词
猕猴桃
软腐病
菌种鉴定
植物精油
香芹酚
绿色防控
kiwifruit
soft rot
pathogens identification
plant essential oil
carvacrol
green prevention and control