Box-Behnken响应面法优化高良姜挥发油的包合工艺研究

Optimization for Inclusion Process of Alpinia Officinarum Hance Volatile Oil by Box-Behnken Response Surface Methodology

目的优化胶体磨法制备高良姜挥发油β-环糊精(β-cyclodextrin,β-CD)包合物的工艺。方法在单因素试验基础上采用Box-Behnken响应面法,以包合物得率和包合率为评价指标,考察β-CD与高良姜挥发油的比例、水与β-CD的比例以及包合时间对包合工艺的影响;通过红外光谱法和显微影像法初步验证高良姜挥发油β-CD包合物的生成。结果高良姜挥发油的最佳包合工艺为β-CD与高良姜挥发油的比例为10∶1,水与β-CD的比例为5∶1,包合时间为45 min。结论胶体磨法包合高良姜挥发油合理、可行。

OBJECTIVE To optimize the preparation process of Alpinia officinarum Hance volatile oil wrapped withβ-cyclodextrin(β-CD)using the colloid mill method.METHODS With yield of inclusion compound and inclusion rate as evaluation indexes,the single factor test combining Box-Behnken response surface methodology was used to study the effects of the ratio ofβ-CD to Alpinia officinarum Hance volatile oil,the ratio of water toβ-CD,and inclusion time on the inclusion process.Inclusion complexes were verified by IR and microscopic image.RESULTS The optimized parameters of inclusion were as follows:the ratio ofβ-CD to Alpinia officinarum Hance volatile oil was 10∶1,the ratio of water toβ-CD was 5∶1,and the inclusion time was 45 min.CONCLUSION The inclusion of Alpinia officinarum Hance volatile oil by colloid mill is reasonable and feasible.