香蕉MaHY5基因密码子的偏好性分析

Analysis of Codon Bias of MaHY5 Gene in Banana

为了解香蕉MaHY5基因密码子的使用特性,本研究利用生物信息学工具,分析了香蕉MaHY5基因的密码子使用偏好性,并分别与其它31个物种的HY5基因以及模式生物基因组进行比较。结果显示,香蕉MaHY5基因密码子选择偏好性弱且偏好以G/C结尾;自然选择的作用对香蕉MaHY5密码子偏性影响最大;多数双子叶植物HY5密码子可能偏好A/T结尾,而单子叶植物HY5密码子可能偏好G/C结尾;基于HY5的CDS序列聚类结果比密码子使用频率分类结果更能反映物种间的进化规律;大肠杆菌原核表达系统更适用于香蕉MaHY5的异源表达试验,拟南芥可能为香蕉MaHY5转基因的最为理想受体。本研究分析了香蕉MaHY5基因及其他物种中HY5基因的密码子偏好性与进化关系,同时筛选最适合MaHY5基因异源表达宿主及遗传转化受体,为深入探究MaHY5基因的功能提供理论基础。

In order to understand the codon usage bias of MaHY5 gene in banana,the HY5 gene of banana was analyzed by Bioinformatics software,and then compared with another HY5 gene of 31 plant species and genomes of model organisms,respectively.The results showed that the codon bias levels of HY5 in banana were weak,and it was bias toward the synonymous codons with G and C at third codon position,and the effect of natural selection on banana MaHY5 codon bias was the greatest.Most dicotyledonous plants might prefer codons ending with A and T,while monocotyledonous plants might prefer codons ending with codon G and C.CDS sequence clustering results based on HY5 can better reflect the evolution of species than codon frequency classification results.E.coli prokaryotic expression system was more suitable for heterogeneic expression test of banana MaHY5,Arabidopsis thaliana may be the most ideal receptor for banana MaHY5 transgenic.The study analyzed the codon bias and evolutionary relationship of the banana MaHY5 gene and other species of HY5 gene,and screened the most suitable heterologous expression host and genetic transformation receptor of MaHY5 gene,which provided a theoretical basis for further exploration of the function of MaHY 5 gene.