番茄果皮开裂的转录组分析

Tomato Pericarp Cracking by Transcriptome Analysis

果皮开裂是造成番茄果实贮藏品质大幅度下降的关键因素。通常,果实开裂受到高温、高湿等外界条件与遗传因素(形状,大小和果皮厚度等)的双重调控。果皮开裂关键调控基因的克隆对优良番茄品种的选育至关重要,有望从源头解决番茄果实开裂的问题。本研究利用显微技术观察果皮开裂后植物细胞组织的变化,发现中果皮薄壁细胞和维管组织受到显著破坏。利用Illumina Hiseq平台对开裂前后的组织进行深度测序,发现果皮开裂后整体植物基因表达数量减少,其中上调植物基因数目1 073个,下调基因数目为972个;通过GO、KEGG和KOG对所有差异表达Reads进行功能分类和富集分析,发现果实开裂与碳代谢、次生物质代谢、信号转导及刺激响应等生命活动密切相关。本研究从细胞和转录水平解析了番茄果皮开裂的机理,也为优质番茄育种提供了良好的理论基础。

Fruit cracking is one of the critical factors affecting the fruit quality and market value. It always results from environmental factors, such as high temperature and humidity, and genetic factors including size, shape,pericarp thickness. In order to decrease the fruit cracking, research tried to find plant genes regulating tomato pericarp dehiscence. Compared to no cracking fruits, parenchymatous cells of pericarp and fibrovascular tissue of cracking fruits are both destroyed by dehiscence through microexamination. Meanwhile, the number of clean reads decreased after tomato fruit cracking examined by Illumina Hiseq platform sequencing. It was found that 1 073 plant genes are up-regulated and 972 genes are down-regulated. Among these genes, 7 highest and 5 lowest expressed ones were selected. However, their encoding proteins had no conserved regions. Then, different expressing genes(DEGs) were annotated through GO, KEGG and KOG. It was found that DEGs are involved in metabolic process(Including carbon metabolism, secondary metabolites) and regulated process(Including signal transduction, stimuli-responsive). These results would provide reference for parsing genetic factor of fruit cracking and selection of plant genes suppressing percarp dehiscence.