样本混合检测在COVID-19诊断中的应用研究

Research on mixed sample detection in the diagnosis of COVID-19

目的研究样本混合检测对COVID-19诊断的影响,探索最佳的混检方式,为疫情防控和相关方案的制定提供科学依据。方法选取包含不同Ct值的阳性样本与已知阴性样本进行1、2、5、10、20、30、40、50、100人份混合,通过实时荧光RT-PCR法检测混合样本Ct值,分析混检对实验结果的影响,探讨最佳混检方法。结果样本混检后,Ct值依次递增,Ct值<30的阳性样本在100份样本混检时可被检出,Ct值在30~35的的阳性样本在50份混检时无法检出,而Ct值>35的样本在5份混检时无法检出。结论样品混检时宜采用"单采混检"方式,采用不多于5个样本均分体积取样混检,可有效提高检测效率,降低漏检率。

Objective To study the effect of mixed sample detection in the diagnosis of COVID-19,to find the best method of the detection,and to provide scientific data for prevention and control of COVID-19.Methods We Chose the positive samples with different Ct values,mixed with the following numbers of negative samples:1,2,5,10,20,30,40,50 and 100 and pooled,and then detected the Ct values of mixed samples by real-time RT-PCR.We analyzed the influence of mixed detection on experimental result,and found the best mixed detection method.Results After the mixed samples were detected,the positive samples with Ct<30 could be detected in 100 samples,the positive samples with Ct between 30 and 35 could not be detected in 50 samples,and the samples with Ct>35 could not be detected in 5 samples.Conclusions The method of"single sampling and mixed testing"should be adopted in the mixed sample detection,and no more than 5 samples should be sampled and mixed by volume,which can effectively improve the detection efficiency and reduce the rate of missed detection.