IL-23在慢性乙型肝炎不同免疫分期中的表达特点及意义

The expression and function of interleukin-23 in different immune phases of chronic hepatitis B patients

目的探讨慢性乙型肝炎(chronic hepatitis B,CHB)患者不同免疫分期中白细胞介素-23(interleukin-23,IL-23)的表达及其对相关细胞因子、基因的影响,探讨IL-23在CHB免疫进展、免疫调节中的作用。方法采用回顾性研究方法,纳入CHB患者197人(包括免疫耐受期患者16人、免疫清除期患者106人、免疫控制期患者59人、再活动期患者16人)及健康对照者26人。采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测血浆中IL-23、IL-17、TGF-β、IL-10、IL-21含量,实时荧光定量PCR(real time quantitative PCR,RT-qPCR)检测外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中ROR-γt mRNA、Foxp3 mRNA表达量。SPSS 23.0分析实验数据。结果CHB患者血浆IL-23、IL-17、IL-21水平、PBMCs ROR-γt mRNA表达水平均高于健康对照组(P<0.05)。CHB患者血浆IL-23与血清ALT、AST、GGT、TBil水平、血浆IL-17、IL-21蛋白水平、PBMCs ROR-γt mRNA、Foxp3 mRNA表达均呈明显正相关(P<0.05)。血浆IL-23、IL-17蛋白及ROR-γt mRNA表达水平在CHB不同免疫分期中存在统计学差异(P<0.05)。结论IL-23可以通过影响相关细胞因子及基因的表达,在CHB不同免疫分期的进程中发挥重要作用。

Objective To determine the expression of interleukin(IL)-23 and its influence on the associated cytokines and genes in different immune phases of chronic hepatitis B(CHB)patients.Methods Our study included 197 patients with chronic hepatitis B(including 16 for immune tolerance phase,106 for immune clearance phase,59 for immune control phase and 16 for reactivation phase).The level of IL-23、IL-17、TGF-β、IL-10、IL-21 in plasma was detected by enzyme-linked immunosorbent assay(ELISA).The mRNA expression level of ROR-γt、Foxp3 was detected by real time quantitative PCR(RT-qPCR).The data were analyzed with IBM SPSS Statistics 23.0 software.Results The level of IL-23、IL-17、IL-21、ROR-γt mRNA in CHB patients is significantly higher than normal controls(P<0.05).The level of IL-23 is positively correlated with ALT、AST、GGT、TBil、IL-17、IL-21 in plasma and ROR-γt mRNA、Foxp3 mRNA level in PBMCs(P<0.05).The expression of IL-23、IL-17 and ROR-γt mRNA are different between different immune phases(P<0.05).Conclusions IL-23 might play an important role on the different immune phases in chronic HBV infection through influencing associated cytokines and genes.