2015年福建省莆田市G基因型腮腺炎病毒全基因组序列测定及分析

Genetic anglysis of genotype G mumps virus based on its complete genome sequence in Putian city,Fujian province,2015

目的了解福建省莆田市2015年G基因型腮腺炎病毒(mumps virus,MuV)全基因组序列特征。方法选择福建省莆田市2015年分离得到的2株腮腺炎流行株进行研究,命名为MuV15-01和MuV15-23。采用逆转录-聚合酶链式反应(reverse transcription polymerase chain reaction,Rt-PCR)对分离株全基因组进行扩增,并对产物进行测序拼接,结合GenBank下载的不同基因型腮腺炎参考株及现有疫苗株序列比对分析分离株基因序列遗传差异及抗原位点变异情况。结果福建省莆田市MuV分离株基因全长15384个核苷酸,与其它基因型毒株相比,全基因组核苷酸差异在3.7%~6.0%之间,其中与A基因型(疫苗株)的遗传差异最大,与N和B基因型的遗传差异最小;在基因组编码7种病毒蛋白核苷酸序列上,SH基因变异最大,M和L基因最为保守;在N基因和HN基因已知抗原相关位点上分别存在7和11个氨基酸位点变异;相比于疫苗株福建分离株在HN基因的464位点上多增加了一个N-糖基化位点。结论现流行于福建省莆田市的G基因型MuV与腮腺炎其他基因型及疫苗株之间在全基因上存在较大的差异,提示需要进一步加强腮腺炎流行株与疫苗株之间的遗传差异性监测,为更好的腮腺炎防控提供科学依据。

Objective To analyze the complete genome sequence characteristics of genotype G mumps virus isolated from Putian city,Fujian province in 2015.Methods Two mumps virus strains isolated in Putian city,Fujian province in 2015 were selected for the study and named as MuV15-01 and MuV15-23.The whole genome of the isolated strains was amplified by reverse transcription-polymerase chain reaction(Rt-PCR).The genetic differences and antigen site variation of the isolated strain were analyzed by comparing the sequences of vaccine strain and other genotypes strains.Results The total length of genome of MuV isolates was 15,384 nucleotides.The nucleotide differences between isolates and other genotypes of mumps virus based on the whole genome ranged between 3.7%and 6.0%.The maximum and minimum difference was found between isolates and genotype A(the vaccine strains),and genotype B to N strains,respectively.Among the nucleotide sequences encoding different viral proteins,SH gene had the largest variation,while M and L genes were the most conserved.There were 7 and 11 amino acid mutations in the known antigen related sites of N gene and HN gene,respectively.Compared with the vaccine strain,the isolated strain added an additional N-glycosylation site to the 464 site of the HN gene.Conclusions Genetic variations were detected in the epidemic mumps virus strains in Putian,Fujian,compared with other mumps genotypes and vaccine strains based on the whole genome.These resluts indicate that the monitoring for genetic differences between epidemic mumps strains and vaccine strains should be further strengthened.