液相色谱-串联质谱法测定禽蛋中β-内酰胺类药物残留

Determination of β-lactam in poultry eggs by liquid chromatography-tandem mass spectrometry

目的建立液相色谱-串联质谱检测禽蛋中药物残留的方法。方法禽蛋用80%乙腈水溶液提取,PRiME HLB固相萃取小柱净化,用液相色谱-串联质谱测定,青霉素G-D7内标法定量。结果在0.5~20μg/L的基质匹配标准溶液内,9种β-内酰胺类药物均呈良好线性关系,相关系数均大于0.994。样品中9种β-内酰类药物的定量限为1μg/kg,在1~10μg/kg的添加浓度范围内平均回收率为73%~96%,相对标准偏差小于10%。结论本方法方便快捷准确,适于禽蛋中的9种β-内酰胺类药物残留检测。

Objective To establish a method for the detection of drug residues in poultry eggs by liquid chromatography-tandem mass spectrometry. Methods Poultry eggs were extracted with 80% acetonitrile aqueous solution, purified with PRiME HLB solid phase extraction column, determined by liquid chromatography-tandem mass spectrometry, and quantitated by penicillin G-D7 internal standard method. Results In a matrix matching standard solution of 0.5 to 20 μg/L, the 9 kinds of β-lactam showed good linear relationship. The correlation coefficients were all greater than 0.994. The quantitative limit of 9 kinds of β-lactam drugs in the sample was 1 μg/kg, and the average recovery was 73%-96% with the relative standard deviation of less than 10% in the range of 1-10 μg/kg. Conclusion This method is convenient, fast and accurate for the detection of 9 β-lactam residues in poultry eggs.