应用篮式生物反应器在BSL-3实验室进行病毒培养的评估

Evaluation of virus cultivation in BSL-3 laboratory by basket bioreactor

目的对篮式生物反应器在BSL-3实验室进行高致病性病毒培养的风险点加以评估,通过对病毒接种、培养、收获3个阶段环境中病毒浓度的监测判断其在BSL-3实验室病毒培养的安全性。方法用灭菌的拭子在病毒的接种、培养、收获3个阶段对篮式生物反应器的罐体及连接处进行涂抹采样,同时在安全柜中,用AirPort MD8空气采样器对病毒的接种和收获操作过成进行空气采样,并且对在3个阶段操作后,实验人员的个人防护装备进行涂抹采样,3种方式进行监测。结果在病毒接种、培养、收获3个阶段,篮式生物反应器的罐体及连接处采样病毒检测结果均为阴性,在病毒接种和收获操作过程中的空气采样病毒检测结果均为阴性,实验人员个人防护装备采样的病毒检测结果均为阴性。结论在BSL-3实验室采用篮式生物反应器进行病毒培养的方法是安全可靠的,通过检测结果显示其在整个操作过程中,未有病毒的泄露,进而确保了实验人员和实验室内环境的生物安全。

Objective The risk points of highly pathogenic virus culture in BSL-3 laboratory in basket bioreactor were evaluated,and the safety of virus culture in BSL-3 laboratory was judged by monitoring virus concentration in three stages of virus inoculation,culture and harvest.Methods Smear samples were taken from the tank and joints of the basket biorereactor at three stages of virus inoculation,culture and harvest with sterilized swabs.At the same time,in the biological safety cabinet,air samples were taken for virus inoculation and harvesting operations using AirPortMD8 air sampler,and in the three stages after operation,the personal protective equipment of researchers was to apply sampling,with three ways to monitoring.Results During the three stages of virus inoculation,culture and receipt,the virus test result of samples taken in the tank and the junction of the basket bioreactor were all negative,the virus test result of air samples taken during the operation stage of inoculation and receipt were all negative,and the virus test result of samples taken in the personal protective equipment of the experimenter were all negative.Conclusions The basket bioreactor method for virus culture in BSL-3 laboratory is safe and reliable,and the test result show that there is no virus leakage during the whole operation process,thus ensuring the biosafety of experimenter and laboratory environment.