几种缓冲溶液简介及应用

Introduction and Application of Several Buffer Solutions

缓冲溶液是许多生化反应、生产、实验过程中的重要溶液,对稳定溶液的酸碱度有重要的影响。以磷酸盐缓冲溶液、硼酸盐缓冲溶液、三羟甲基氨基甲烷(简称Tris)-HCl缓冲溶液、4-羟乙基哌嗪乙磺酸(简称HEPES)缓冲溶液为例,介绍缓冲溶液的组成、缓冲原理及其在生物化学方面的应用。为初学者深入了解缓冲溶液和在实验中选择合适的缓冲溶液提供参考。

The buffer solution is an important solution in many biochemical reactions,production and experiments,which has a significant effect on stabilizing the pH value of the solution.The phosphate buffer solution,borate buffer solution,Tris-HCl buffer solution and HEPES buffer solution are taken as the examples to introduce the composition,principium and application in biochemistry of the buffer solution.It also provides some references for the beginners to further understand the buffer solution and to choose buffer solution properly in experiments.