红凤菜不同器官中黄酮类和奎宁酸类成分的HPLC法测定

Determination of components of flavonoids and quinic acids in different organs of Gynura bicolor by HPLC method

红凤菜〔Gynura bicolor(Willd.)DC.〕隶属于菊科(Asteraceae)菊三七属(Gynura Cass.),为多年生草本植物,可作为药食两用植物被人们利用。红凤菜全草均可入药[1],含有丰富的黄酮类、奎宁酸类和萜类等次生代谢产物,具有良好的止血、降血糖和抗氧化等药理活性[2-6]。目前,从红凤菜地上部分已经分离鉴定出多种酚酸类[2,7]和黄酮类成分[8-9],并对其总黄酮含量进行了分析[10],但对红凤菜不同器官中黄酮类和酚酸类成分的差异尚缺乏深入了解。

Contents of rutin,isoquercitrin,kaempferol-3-O-rutinoside,neochlorogenic acid,chlorogenic acid,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in root,stem and leaf of Gynura bicolor(Willd.)DC.were determined by using HPLC method.The results show that all 7 components can be detected in leaf of G.bicolor,and their contents are 0.279-4.804 mg·g-1,in which,contents of chlorogenic acid and isoquercitrin are relatively high,while contents of rutin and neochlorogenic acid are relatively low;only chlorogenic acid,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid are detected in its root and stem,and their contents are all significantly lower than those in leaf.It is recommended that leaf of G.bicolor can be used as the medicinal part based on the measured result.