湿疹患者外周血白细胞介素-21和B细胞淋巴瘤因子-6 mRNA的表达和临床意义

Expression and clinical significance of IL-21 and Bcl-6 mRNA in peripheral blood of eczema patients

目的:探讨滤泡性辅助性T细胞的主要效应因子白细胞介素(IL)-21和B细胞淋巴瘤因子(Bcl)-6在湿疹患者外周血中的表达及意义。方法:采用实时荧光定量反转录聚合酶链式反应(RT-PCR),检测60例湿疹患者和40例健康者外周血IL-21和Bcl-6 mRNA的表达量并进行临床分析。结果:湿疹患者外周血中IL-21和Bcl-6 mRNA的表达水平明显低于健康者(P<0.05),与湿疹面积及严重度指数(EASI)评分及嗜酸性粒细胞计数呈负相关(P<0.001),与患者年龄、白细胞计数无明显相关性(P>0.05)。不同性别的湿疹患者外周血中IL-21和Bcl-6 mRNA表达差异均无统计学意义(P>0.05)。不同分型湿疹患者外周血中IL-21和Bcl-6 mRNA的表达水平差异均有统计学意义,其中急性湿疹组低于亚急性湿疹组和慢性湿疹组,亚急性湿疹组低于慢性湿疹组(P<0.05)。经过综合治疗2周及4周后,湿疹患者外周血中IL-21和Bcl-6 mRNA的表达水平比治疗前及治疗后2周均升高,差异均有统计学意义(P<0.05)。湿疹患者外周血中IL-21和Bcl-6 m RNA的表达呈正相关(P=0.001)。结论:湿疹患者中存在IL-21和Bcl-6 mRNA的表达异常,提示这2个细胞因子可能参与了湿疹的发病过程。

Objective: To investigate the expression and clinical significance of IL-21 and Bcl-6 mRNA in peripheral blood of patients with eczema. Methods: The expression levels of IL-21 mRNA and Bcl-6 mRNA in peripheral blood of 60 eczema patients and 40 healthy people were detected by RT-PCR for clinical analysis. Results: The expression levels of IL-21 mRNA and Bcl-6 mRNA in peripheral blood of eczema patients were significantly lower than those in healthy group(P<0.05), and were negatively correlated with EASI score and eosinophils(P<0.001), while these were not significant correlated with age and white blood cell count(P>0.05). There was no significant difference in the expression of IL-21 mRNA and Bcl-6 mRNA in the peripheral blood of eczema patients of different genders(P>0.05). The expression levels of IL-21 mRNA and Bcl-6 mRNA in pe ripheral blood of patients with different types of eczema were significantly different. The expression levels of IL-21 m RNA and Bcl-6 mRNA in acute eczema were lower than those in subacute and chronic eczema(P<0.05). After 4 weeks of comprehensive treatment, the levels of IL-21 mRNA and Bcl-6 mRNA in peripheral blood of eczema patients were significantly higher than those before and after 2 weeks treatment(P<0.05). The expression of IL-21 mRNA was positively correlated with the expression of Bcl-6 mRNA in peripheral blood of eczema patients(P=0.001). Conclusion: The abnormal expression of IL-21 m RNA and Bcl-6 mRNA in patients with eczema suggested that these two cytokines might be involved in the pathogenesis of eczema.