活化T细胞核因子在新疆哈萨克族不同级别高血压患者中的表达

Expression of Nuclear Factor of Activated T Cells in Patients with Different Grades of Hypertension in Xinjiang Kazakh Nationality

目的研究活化T细胞核因子(NFAT)及其相关分子在新疆哈萨克族不同级别高血压患者外周血T淋巴细胞中的表达水平变化。方法选取就诊于新疆医科大学第一附属医院高血压病房未经药物干预的新疆哈萨克族高血压1级组、2级组和3级组为试验组,血压正常的哈萨克族健康者为对照组,各30例,各组男女比例相等。运用RT-qPCR技术和Western blotting技术分别检测外周血T淋巴细胞NFAT、白介素-17基因和NFAT蛋白表达。结果与对照组相比,高血压1级组、2级组和3级组的NFAT mRNA和蛋白以及白介素-17 mRNA相对表达量明显增加(P<0.05)。NFAT mRNA表达水平与高血压2级组和高血压3级组均存在正相关关系(P<0.01);NFAT蛋白和白介素-17 mRNA表达水平与3个不同级别高血压组均存在正相关关系(P<0.05)。结论NFAT在新疆哈萨克族不同级别高血压患者外周血T淋巴细胞上的表达随血压水平的升高而增加并呈正相关关系。

Objective To study the expression level of nuclear factor of activated T cells(NFAT)and its related molecules in T lymphocytes of peripheral blood from patients with different grades of hypertension in Xinjiang Kazakh nationality.Methods The Kazakh patients hospitalized in hypertensive ward of The First Affiliated Hospital of Xinjiang Medical University with grade 1,grade 2 and grade 3 hypertension without drug intervention were selected as experimental group,and the Kazakh healthy people with normal blood pressure as control group.30 cases with same proportion of gender were divided into each group.RT-qPCR and Western blotting were used to detect the expression of NFAT,IL-17 gene and NFAT protein in T cells,respectively.Results The relative expression of NFAT mRNA,protein and IL-17 mRNA in grade 1,grade 2 and grade 3 hypertension group was significantly increased compared with control group(P<0.05).The expression of NFAT mRNA was positively correlated with grade 2 and grade 3 hypertension group(P<0.01).The expression levels of NFAT protein and IL-17 mRNA were both positively correlated with three grade hypertension groups(P<0.05).Conclusion The expression of NFAT in T lymphocytes of peripheral blood from Xinjiang Kazakh patients with different grades of hypertension was increased and positively correlated with elevated blood pressure.