基于GC-MS的2型糖尿病血浆代谢组学研究

Plasma metabolomics of type 2 diabetes mellitus based on GC-MS

目的应用代谢组学技术对2型糖尿病(T2DM)患者和健康人群进行对比研究,探讨T2DM的致病机制。方法采用气相色谱-质谱联用(GC-MS)结合多种多元统计分析方法对2018年6—7月黑龙江省佳木斯市中心医院肾内科收集的健康组(60名)、T2DM组(100例)的血浆样品进行分析。结果建立了基于GC-MS的T2DM血浆代谢组学分析方法,确定了血浆中L-丙氨酸、乳酸、L-缬氨酸等10种差异代谢物。结论基于代谢组学的GC-MS检测分析得出T2DM患者与健康组代谢模式有明显差异,与糖脂代谢、氨基酸代谢等代谢途径相关。

Objective To conduct a study of type 2 diabetes mellitus(T2DM)patients and healthy people by metabolomics techniques,to explore the pathogenesis of T2DM.Methods Gas chromatography-mass spectrometry(GC-MS)combined with multiple multivariate statistical analysis methods was used to analyze plasma samples from healthy group(60 people)and the T2DM group(100 cases)in the Department of Nephrology,Jiamusi Central Hospital of Heilongjiang Province from June to July 2018.Results The method of plasma metabonomic analysis of T2DM based on GC-MS was established.Ten different metabolites such as L-alanine,lactic acid and L-valine in plasma were determined.Conclusion Based on metabolomic GC-MS analysis showed that the metabolic pattern of T2DM patients is significantly different from that of healthy group,which is related to metabolic pathways such as glucose and amino acid metabolism.