尖海龙共附生真菌Talaromyces amestolkiae的化学成分

Chemical constituents of Talaromyces amestolkiae, a symbiotic fungus of Syngnathus acus

对尖海龙共附生真菌Talaromyces amestolkiae的化学成分进行研究.采用固体培养基对该菌株进行培养,利用硅胶柱、Sephdex-LH-20凝胶柱、MCI柱、制备色谱等方法对化学成分进行分离纯化,并运用质谱、核磁共振等方法进行结构鉴定.共分离得到10个化合物,分别为methyl 2-formyl-4-methylfuro[3,2-b]pyrrole-5-carboxylate(1)、4-甲基苔黑酚(2)、麦角甾-5,7,22-三烯-3-醇(3)、3-甲氧基苯酚(4)、thailandolide B(5)、aspergillumarin B(6)、(R)-3-(3-hydroxypropyl)-8-hydroxy-3,4-dihydroisocoumarin(7)3-hydroxymethyl-6,8-dimethoxycoumarin(8)、penicifuran D(9)、penicifuran C(10).其中化合物1为首次分离得到的天然产物,化合物2-10均首次从T.amestolkiae中分离.(参33)

This study focused on the chemical constituents of Talaromyces amestolkiae, a symbiotic fungus obtained from Syngnathus acus Linnaeus. Talaromyces amestolkiae was cultured with solid-state fermentation, and its secondary metabolites were separated by silica gel, Sephadex LH-20, MCI column, as well as preparative chromatography. The subsequent compounds were identified by spectral methods. Ten compounds were isolated and identified as methyl 2-formyl-4-methylfuro[3,2-b]pyrrole-5-carboxylate(1), 4-methylorcnol(2), erg osta-5,7,22-trien-3-ol(3),3-methoxyphenol(4), thailandolide B(5), aspergillumarin B(6),(R)-3-(3-hydroxypropyl)-8-hydroxy-3,4-dihydroisocoumarin(7), 3-hydroxymethyl-6,8-dimethoxycoumarin(8), penicifuran D(9), penicifuran C(10). Compound 1 was obtained from natural source for the first time. Compounds 2-10 were isolated from this genus for the first time.