miR-409-3p在卵巢浆液性癌中的表达及对HO-8910细胞生物学特性的影响

Expression of miR-409-3p in ovarian serous carcinoma and its effect on biological behaviors of HO-8910 cells

目的探讨微小RNA-409-3p (microRNA-409-3p或miR-409-3p)在卵巢浆液性癌组织中的表达和临床意义以及对HO-8910细胞生物学特性的影响。方法选取2014年本院手术治疗的40例卵巢浆液性癌组织和20例正常卵巢组织,采用RT-qPCR法检测miR-409-3p在各组织中的表达,分析其与患者临床病理参数之间的关系。进一步分析miR-409-3p在人卵巢浆液性癌细胞HO-8910、SKOV3和卵巢表皮细胞IOSE80中的表达差异;对人卵巢浆液性癌HO-8910细胞进行瞬时转染miR-409-3p模拟物(mimics),分别采用CCK8细胞增殖实验、流式细胞术和Transwall侵袭实验检测过表达miR-409-3p对HO-8910细胞增殖、凋亡和侵袭能力的影响。结果 miR-409-3p在卵巢浆液性癌组织中的表达较卵巢正常组织显著降低(P<0.001),低表达的miR-409-3p与患者国际妇产科联合会(international federation of gynecology and obstetrics, FIGO)分期和大网膜转移存在显著相关性(P<0.05),与患者年龄、组织学分级和CA125指标无显著相关(P>0.05);miR-409-3p低表达组的总生存期显著低于高表达组(P<0.05)。与IOSE80细胞相比,miR-409-3p在HO-8910和SKOV3细胞中表达减少。与空白对照组和阴性对照组相比,转染miR-409-3p mimics后HO-8910细胞增殖和侵袭能力明显降低,细胞凋亡比例明显增加(P<0.05)。结论 miR-409-3p在卵巢浆液性癌组织和细胞中呈低表达状态,其表达水平与患者FIGO分期、大网膜转移和总生存期有关,过表达miR-409-3p显著抑制HO-8910细胞的增殖和侵袭能力,促进细胞凋亡。

Objective To investigate the expression of miR-409-3 p in ovarian serous carcinoma and its correlation with the clinicopathological characteristics of the patients and observe the effect of miR-409-3 p overexpression on the proliferation,invasion and apoptosis of HO-8910 cells.Methods We collected 40 tissue specimens of ovarian serous carcinomas and 20 normal ovarian tissues from patients undergoing surgeries for ovarian serous carcinoma in our hospital between January,2014 and December,2014.We examined the expression of miR-409-3 p in the tissue specimens using RT-qPCR and explored the correlation of miR-409-3 p with the clinicopathological parameters of the patients.We also detected the expression of miR-409-3 p in 2 ovarian serous carcinoma cell lines(HO-8910 and SKOV3)and a human ovarian epithelial cell line(IOSE80).The effects of transient transfection with miR-409-3 p mimics on the proliferation,apoptosis and invasion of HO-8910 cells were observed using CCK8 assay,flow cytometry and Transwell invasion assay,respectively.Results The expression of miR-409-3 p was significantly lower in ovarian serous carcinoma tissues than in normal ovarian tissues(P<0.001)and showed significant correlations with the FIGO stage(Ⅲ/Ⅳ)and omental metastasis of the tumor(P<0.05),but not with the patients’age,histological grade or CA125 level(P>0.05).The patients with low miR-409-3 p expressions had significantly shorter overall survival than those with high miR-409-3 p expression levels(P<0.05).The expression of miR-409-3 p was also significantly lower in HO-8910 and SKOV3 cells than in IOSE80 cells,and overexpression of miR-409-3 p significantly suppressed the proliferation and invasion and promoted the apoptosis of HO-8910 cells(P<0.05).Conclusion miR-409-3 p is lowly expressed in ovarian serous carcinoma and its expression level is significantly correlated with the FIGO stage,omental metastasis and overall survival of the patients.Overexpression of miR-409-3 p inhibits the proliferation and invasion and promotes apoptosis of HO-8910 cells,suggesting the value of miR-409-3 p as a potential prognostic indicator and a therapeutic target for ovarian serous carcinomas.