基于UHPLC-Q-TOF/MS的预知子入血成分及其体内代谢研究

In-vivo Studies of the Metabolites of Akebiae Fructus Extract Based on UHPLC-Q-TOF/MS Method

目的研究预知子提取物的主要化学成分及其入血成分,分析其在大鼠体内(血浆、尿液、粪便、胆汁)的代谢途径。方法采用超高效液相色谱与飞行时间质谱联用(UHPLC-Q-TOF/MS)技术,结合对照品质谱裂解规律及文献报道,对预知子提取物的化学成分进行鉴定,总结其质谱裂解规律,并应用于体内代谢物的分析鉴定。结果建立了大鼠灌胃给药预知子提取物后血浆、尿液、粪便及胆汁中活性物质及其代谢物的分析方法,共鉴定化合物20个和代谢物28个(三萜皂苷的代谢物23个,绿原酸类化合物的代谢物5个)。结论预知子提取物中的三萜皂苷和绿原酸类化合物均有入血,其中三萜皂苷的体内代谢以还原、甲基化、去甲基化以及羟基化为主,绿原酸类化合物体内代谢方式主要为甲基化和葡萄糖醛酸化。

Objective To analyze and identify the compounds and its metabolites of Akebiae fructus extract in rats for exploring its metabolism mode in vivo.Methods UHPLC-Q-TOF/MS was applied for the analysis of the compounds in Akebiae fructus extract as well as their metabolites.The cleavage patterns of the compounds in vitro were analyzed by referring to literatures and the cleavage of reference substances,and then applied to the identify metabolites in vivo.Results Based on the mass spectrometry fragmentation rules of the compounds in Akebiae fructus extract,a method was established for the identification of metabolites in plasma,urine,feces and bile after administration.Twenty compounds of Akebiae fructus were identified and 28 metabolites were detected in the biological samples(in which,23 were metabolites of triterpenoid saponins,and 5 were those of chlorogenic acids).Conclusion The metabolic pathways included hydrogenation,methylation,hydroxylation,demethylation for the metabolites of triterpenoid saponins;and methylation,glucuronidation for the metabolites of chlorogenic acids.