摘要
目的建立高效液相色谱(HPLC)法同时测定玳玳花中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、桔皮素的含量,为其质量控制提供参考。方法采用Shim-Pack GIST C18色谱柱(150 mm×4.6 mm,5.0μm);以0.1%磷酸水(A)-乙腈(B)为流动相,梯度洗脱;流速:1.0 mL·min-1;检测波长:283 nm。结果玳玳花中5个成分在考察的浓度范围内,浓度与峰面积之间呈良好的线性关系(r>0.999);回收率均在100.13%~103.98%范围内,RSD均小于3.0%。聚类分析可直观地把4个不同产地的样品进行分类。结论该方法简便、准确,可用于比较不同来源玳玳花的差异,并为其质量标准建立提供依据。
Objective To establish a UHPLC method for simultaneous determination of five components(Narirutin,Naringin,Hesperidin,Neohesperidin and Tangeretin)in Citrus aurantium L.var.amara Engl.,in order to provide evidences for its quality control.Methods The separation was performed on a Shim-Pack GIST C18 column(150 mm×4.6 mm,5.0μm)with gradient elution using 0.1%phosphoric acid(A)and acetonitrile(B)as mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength was 283 nm.Results All calibration curves were linear(r>0.999)over the tested ranges.The average recoveries ranged from 100.13%to 103.98%with RSD values below 3.0%.Statistics software can intuitively classify samples from four different origins.Conclusion The established method is simple,fast and with high precision,which can be used to compare the differences of different sources and provide references for establishment of quality standard for Citrus aurantium L.var.amara Engl.
作者
黄玉林
李梦楚
陈新滔
吴东
龚明
曾燕斌
龚建平
温芝琪
黄文平
HUANG Yulin;LI Mengchu;CHEN Xintao;WU Dong;GONG Ming;ZENG Yanbin;GONG Jianping;WEN Zhiqi;HUANG Wenping(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004 Jiangxi,China;Solid Preparation of Traditional Chinese Medicine Manufacturing Technology National Engineering Center,Nanchang 330006 Jiangxi,China;Nanchang First Hospital,Nanchang 330000 Jiangxi,China;Jiangxi Jiashu Health Management Co.,Ltd.,Nanchang 330000 Jiangxi,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2020年第12期1478-1482,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家重大研究发展计划项目(2017YFC1702906)
江西省科技厅应用研究培育计划项目(2018BBG78053)。
关键词
玳玳花
高效液相色谱法
芸香柚皮苷
柚皮苷
主成分分析
Citrus aurantium L.var.amara Engl.
HPLC
narirutin
naringin
principal component analysis