摘要
目的:建立淫羊藿配方颗粒UPLC特征图谱及含量测定方法,为淫羊藿配方颗粒的质量控制提供依据。方法:采用HPLC法及紫外-可见分光光度法分别测定淫羊藿配方颗粒中淫羊藿苷和总黄酮含量,并建立淫羊藿配方颗粒UPLC特征图谱。结果:18批淫羊藿配方颗粒中淫羊藿苷和总黄酮的含量范围分别为3.2%~5.9%、19.2%~31.6%;UPLC特征图谱确定了5个共有峰,通过与对照品比较,分别为朝藿定A、朝藿定B、朝藿定C、淫羊藿苷和宝藿苷-Ⅰ,对18批淫羊藿配方颗粒进行相似度比较,其相似度均大于0.900。结论:本研究建立的方法精密度、重复性和稳定性良好,可用于淫羊藿配方颗粒的质量控制和评价。
Objective:To establish the UPLC characteristic spectrum and content determination method of Yinyanghuo formula granules,and provide a basis for the quality control of Yinyanghuo formula granules.Methods:HPLC and ultraviolet-visible spectrophotometry were used to determine the contents of icariin and total flavonoids in Yinyanghuo formula granules,and the UPLC characteristic spectrum of Yinyanghuo formula granules was established.Results:The contents of icariin and total flavonoids in 18 batches of Yinyanghuo formula granules ranged from 3.2% to 5.9%,19.2% to 31.6%,respectively.UPLC characteristic spectrum identified 5 common peaks.By comparing with the reference,they are epimedin A,epimedin B,epimedin C,icariin and baohuoside-I.Comparing the similarity of formula granules,the similarity were all greater than 0.900.Conclusion:The method established in this study has good precision,repeatability and stability,and can be used for the quality control and evaluation of Yinyanghuo formula granules.
作者
张正
陈江平
程学仁
李慧馨
孙冬梅
汪梅
ZHANG Zheng;CHEN Jiang-ping;CHENG Xue-ren;LI Hui-xin;SUN Dong-mei;WANG Mei(Guangdong Yifang Pharmaceutical Co.,Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule Enterprises,Foshan Guangdong 528244,China;Sinopharm Group Tongjitang(Guizhou)Pharmaceutical Co.,Ltd.,Guiyang Guizhou 550000,China)
出处
《中医药导报》
2020年第14期20-23,28,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
广东省省级科技计划项目(科技基础条件建设领域)(2018B030323004)
广东特支计划科技创业领军人才项目(2017TY04R197)。
关键词
淫羊藿配方颗粒
含量测定
淫羊藿苷
总黄酮
UPLC特征图谱
Yinyanghuo formula granules
content determination
icariin
total flavonoids
UPLC characteristic spectrum