肉苁蓉苯乙醇总苷纳米乳在体鼻腔吸收研究

Study on nasal absorption of Cistanche deserticola phenylethanol glycosides nanoemulsion in vivo

该实验考察了肉苁蓉苯乙醇总苷纳米乳的经鼻吸收特性及其影响因素。采用经典的大鼠在体鼻腔循环灌流模型,以吸收速度常数为考察指标,比较不同浓度的肉苁蓉苯乙醇总苷纳米乳与其水溶液的鼻腔吸收特性,并探究制剂pH与吸收促进剂氮酮对肉苁蓉苯乙醇总苷纳米乳鼻腔吸收的影响。结果显示,与含药水溶液组相比,同浓度的肉苁蓉苯乙醇总苷纳米乳的吸收速度常数明显升高(P<0.05),肉苁蓉苯乙醇总苷纳米乳更易被大鼠鼻腔吸收;肉苁蓉苯乙醇总苷浓度对纳米乳经鼻吸收量呈剂量依赖性升高;当纳米乳pH为6.0,氮酮比例为2%时,吸收速度常数最大,促渗效果最好。

To investigate the transnasal absorption characteristics of Cistanche deserticola phenylethanol glycosides nanoemulsion and its influencing factors. With the use of the classic in vivo nasal circulation perfusion model in rats, the absorption rate constant was used as the index to compare the nasal absorption characteristics of C. deserticola phenylethanol glycosides nanoemulsion and its aqueous solution in different concentrations, and to explore the effects of pH value of the preparation and absorption accelerator Azone on the nasal absorption of C. deserticola phenylethanol glycosides nanoemulsion. The results showed that, as compared with the aqueous solution group, the absorption rate constant was significantly higher in C. deserticola phenylethanol glycosides nanoemulsion with the same concentration(P<0.05), and C. deserticola phenylethanol glycosides nanoemulsion was more easily absorbed by the nasal cavity of rats;with the increase of the concentration of C. deserticola phenylethanol glycosides, the transnasal absorption amount of nanoemulsion was also increased in a dose-dependent manner. When the pH value of nanoemulsion was 6.0 and the ratio of Azone was 2%, the absorption rate constant was highest and the effect of promoting infiltration was the best.