病毒载量检测在102例HIV抗体不确定样本诊断中的应用

Application of Viral Load Testing in Diagnosing 102 Cases of Indeterminate HIV Antibody

目的探讨病毒载量检测在HIV抗体不确定样本诊断中的应用价值。方法选取2016年1月至2019年12月期间昆明市第三人民医院门诊及住院102例HIV抗体不确定病例,1周内进行COBAS TaqMan HIV-1 Test v2.0病毒载量检测和CD4细胞计数,2~4周后随访蛋白印迹实验(western blotting,WB)检测,综合比对分析两种方法的检测结果。结果102例HIV抗体不确定病例,病毒载量检测结果大于20 IU/mL的84例样本随访均转阳,2例病毒载量检测结果低于检测下限20 IU/mL,随访结果最终为阳性,病毒载量检测结果为TND的16例病例,随访结果为阴性。结论高精度病毒载量检测对HIV抗体不确定结果能快速准确鉴别,缩短随访复查的时间,提早对病情准确判断,核酸检测作为补充实验可用于艾滋抗体确证试验不确定样本的判定。

Objective To explore the application value of viral load testing in diagnosing HIV antibody indeterminate cases.Methods The COBAS TaqMan HIV-1 Test v2.0 and CD4 cell count in 102 cases of indeterminate HIV antibody from The Third People’s Hospital of Kunming from January 2016 to December 2019 were detected in one week.Patients were followed up to get the blood samples again two to four weeks later for the retest by Western blotting(WB).The results of the two methods were compared.Results A total 102 cases of blood samples showed unclear WB test result,with 86 cases proved to be HIV positive by follow-up WB test,VL test results of 19 samples more than 20 IU/ml and 2 samples below detection limit 20 IU/ml.16 cases got confirmed diagnosis of negative HIV antibody by follow-up WB test,and their VL were all TND.Conclusions HIV antibody detection results can be identificated fastly and accurately by high precision detection of viral load.And the time for follow-up will be.shorten.The disease can be accurately judged early.The National Technical Specification for AIDS Testing(2015 Revision)also states that viral load testing as an effective auxiliary detection method for indeterminate samples of HIV antibody.High-precision detection of viral load is of great significance.