1,25(OH)2D3对猪伪狂犬病病毒诱导小鼠流产的影响

Effect of 1,25(OH)D3 on pseudorabies virus-induced abortion in a mouse model

旨在探明1,25(OH)2D3对猪伪狂犬病病毒(pseudorabies virus,PRV)诱导小鼠流产的影响。采用颈背部皮下注射不同剂量PRV建立PRV诱导小鼠流产模型;通过实时荧光定量PCR检测不同剂量1,25(OH)2D3预处理组和生理盐水预处理组小鼠卵巢和子宫内VDR mRNA的水平及PRV量;利用组织学病理切片技术观察PRV引起的1,25(OH)2D3预处理组和生理盐水处理组小鼠子宫和卵巢的病理变化。结果显示,成功建立了小鼠流产模型,确定了引起小鼠流产的PRV最佳接种剂量为0.35 mL 1 000 TCID50 PRV;用300μg/kg 1,25(OH)2D3预处理,小鼠VDR表达水平最高,能有效抑制病毒的增殖,对PRV感染小鼠流产的抑制作用最强。组织病理学检查发现,相较于生理盐水预处理的PRV感染组,1,25(OH)2D3预处理组小鼠卵泡腔内颗粒细胞增多、红细胞量减少;子宫腔内红细胞数量减少或消失,子宫内膜皱褶增多。结果表明,1,25(OH)2D3能有效抑制PRV增殖,降低PRV诱导的小鼠流产。

In order to analyze the protect effect of 1,25(OH)2D3 against pseudorabies virus-induced abortion in a mouse model.In this study,pregnant mice were inoculated with different dosage of pseudorabies virus(PRV)to establish abortion model.VDR mRNA and PRV titers in the ovary and uterus of mice pretreated with different dosage of 1,25(OH)2D3,were detected with real-time PCR.Pathological sections of ovary and uterus were prepared to examine the pathologic changes.The results showed that the optimum PRV dosage to induce abortion in mice was 0.35 mL 1 000 TCID50,and the highest expression of VDR was induced by 300 μg/kg 1,25(OH)2D3.Pretreated mice with 300 μg/kg 1,25(OH)2D3 can inhibit PRV replication,retard the abortion time and decrease abortion rate,effectively.Histopathological examination further confirmed these results.Compare with the PRV infected mice pretreated with normal saline,the number of red blood cell in uterine cavity of the mice pretreated with 1,25(OH)2D3 was decreased,while granulose cell in follicle cavity and the endometrial fold were increased.In conclusion,1,25(OH)2D3 can inhibit PRV replication and PRV-induced abortion in mice effectively.