Epikutis模型替代法研究大麻叶提取物的抗炎修复功效

Study on repair and anti-inflammatory effects of Cannabis sativa leaf extract by Epikutis model substitution method

为了探索大麻叶提取物的抗炎功效以及对受损皮肤的修复作用,使用3D表皮模型(Epikutis?)进行检测。在实验过程中采用SLS预处理上皮细胞表面24 h后,通过测试组织活力和具有代表性的促炎症因子IL-1α、TNF-α及IL-8观察大麻叶提取物的抗炎功效以及修复作用。通过MTT试验检测组织活力,结果表明大麻叶提取物有效抑制炎性因子IL-1α,TNF-α及IL-8分泌,同时在使用该样品后皮肤模型组织活力明显提升。大麻叶提取物可通过抑制皮肤炎症反应,从而在皮肤屏障损伤方面具有修复作用。

To explore the anti-inflammatory effect and repair effect of Cannabis sativa leaf extract on damaged skin,in this study,an in vitro recombinant 3D epidermal model(Epikutis®)construted by human keratinocytes was used to test.In the experiment,SLS was used to treat the surface of epithelial cells for 24 hours,and the tissue vitality and representative pro-inflammatory factors IL-1α,TNF-αand IL-8 were tested.To observe the anti-inflammatory effect and repairing effect of Cannabis sativa leaf extract,MTT test was used to measure the tissue vitality.The results showed that Cannabis sativa leaf extract could effectively inhibit the secretion of inflammatory factors IL-1α,TNF-αand IL-8,and the vitality of skin model tissue was obviously improved after using this sample,which indicated that Cannabis sativa leaf extract could repair the skin barrier injury by inhibiting the skin inflammatory reaction.