ICP-MS法测定不同产地菊花药材中重金属含量及聚类分析

Determination of heavy metals in the medicinal material chrysanthemum from different producing areas by inductively coupled plasma mass spectrometry and a cluster analysis

目的:建立菊花药材中铅、镉、砷、汞、铜5种有害金属元素的测定方法,并分析不同产地菊花药材重金属的分布情况。方法:采用电感耦合等离子体质谱法(ICP-MS)对不同产地菊花中铅、镉、砷、汞、铜5种重金属及有害元素进行含量测定。结果:铅、镉、砷、汞、铜5种元素的精密度、重复性、稳定性RSD均<3.0%,加样回收率(n=6)在87.9%~105.8%之间。采用系统聚类分析不同产地菊花药材中重金属含量,认为以江苏产地的菊花药材质量较好。结论:本研究建立的ICP-MS具备灵敏度高、准确性好的优点,可用于菊花中5种重金属及有害元素的同时检测,为菊花的安全性评价提供科学依据,并为其他药材的测定提供参考。

Objective: To establish the method for determining the content of five heavy metals(lead,cadmium,arsenic,mercury,and copper) in the medicinal material chrysanthemum,and to investigate the distribution of heavy metals in chrysanthemum from different producing areas.Methods: Inductively coupled plasma mass spectrometry(ICP-MS) was used to determine the content of five heavy metals(lead,cadmium,arsenic,mercury,and copper) and harmful elements in chrysanthemum from different producing areas.Results: The precision,repeatability,and stability of the five heavy metals lead,cadmium,arsenic,mercury,and copper had a relative standard deviation of <3.0% and an average recovery rate(n = 6) of 87.9% ~ 105.8%.The hierarchical cluster analysis was used to analyze the content of heavy metals in chrysanthemum from different producing areas,and the results showed that the medicinal material chrysanthemum from the producing areas in Jiangsu tended to have better quality.Conclusion: ICP-MS established in this study has the advantages of high sensitivity and good accuracy and can be used for the simultaneous determination of five heavy metals and harmful elements in chrysanthemum,providing a scientific basis for the safety evaluation of chrysanthemum and a reference for the determination of other medicinal materials.