基于简单重复序列标记的药用梅品种的身份证构建

Construction of Identification Cards of Medicinal Plum Varieties Based on Simple Sequence Repeats Markers

目的筛选一套适合中国药用梅品种鉴定标准的简单重复序列(simple sequence repeats,SSR),用于构建药用梅品种DNA指纹身份证。方法以30份药用梅农家种为材料,从180对SSR引物中,最终选取16对多态性较高、条带清晰且重复性好的SSR引物对30份药用梅农家种进行扩增。结果30份药用梅共扩增出114个等位基因,平均等位基因数和多态信息含量分别为7.12和0.69,且在16个位点上识别两个具有相同基因型的随机个体的概率估计为5.24×10-17。最终筛选出4对扩增稳定、多态性强的核心引物,可将30个药用梅农家种进行有效区分,建立药用梅种质资源DNA指纹身份证。结论SSR标记技术可以作为药用梅分子身份证构建的有效技术手段。

Objective To screen out a set of simple sequence repeats(SSR)molecular markers which is suitable for the identification criteria of medicinal plum varieties in China and can be used to construct the DNA fingerprint identification cards of medicinal plum varieties.Methods A total of 30 species of medicinal plum were selected,and among 180 pairs of SSR primers,16 pairs with high polymorphism,clear bands,and good repeatability were selected for amplification.Results A total of 114 alleles were amplified in the 30 species of medicinal plum,and the average number of alleles and polymorphism information content were 7.12 and 0.69,respectively.The estimated probability was 5.24×10-17 for identifying two random individuals with the same genotype on 16 loci.Finally,4 pairs of core primers with stable amplification and strong polymorphism were screened out,which effectively distinguished the 30 species of medicinal plum and could be used to establish the DNA fingerprint identification cards of medicinal plum germplasm resources.Conclusion SSR labeling technique can be used as an effective technique for constructing the molecular identification cards of medicinal plum.