常压室温等离子体诱变选育高产脂肪酶解脂耶氏酵母

Breeding of Yarrowia lipolytica with high lipase production by atmospheric room temperature plasma mutagenesis

采用常压室温等离子体(Atmospheric room temperature plasma,ARTP)对产脂肪酶的解脂耶氏酵母菌株YL1进行诱变;通过三丁酸甘油酯平板法、p-NPP法以及酸碱滴定法等筛选得到高产脂肪酶的目标菌株C4,并研究其遗传稳定性。结果表明,解脂耶氏酵母菌株YL1的最佳诱变时间为60s,菌株致死率达97.45%;突变株C4的脂肪酶酶活为13.4 U/mL,较出发菌株提高了82.6%,多代培养后遗传稳定;与出发菌株相比,突变株脂肪酶可使维生素A棕榈酸酯的合成转化率提高36.9%。

A lipase-producing Yarrowia lipolytica strain YL1 was mutagenized by atmospheric room temperature plasma.Tributyrin plate method,p-NPP method and acid-base titration method were used to screen out the target strain C4 with high lipase production,and then its genetic stability was studied.The results showed that the optimal mutagenesis time of Y.lipolytica strain YL1 was 60 s,and the lethality rate of the strain reached97.45%.The lipase activity of the mutant strain C4 was 13.4 U/mL,which was 82.6%higher than the original strain,and it was genetically stable after multiple generations of culture.Compared with the original strain,the mutant strain lipase could increase the synthesis-conversion rate of vitamin A palmitate by 36.9%.