丁香酚对水产品中普罗威登斯菌的抑制作用

Inhibitory effect of eugenol on Providence vermicolain aquatic products

以从自然腐败变质的香辣蟹中分离鉴定的一株普罗威登斯菌(Providencia vermicola)为对象,研究丁香酚对其抑菌作用及其机理。结果表明,丁香酚对普罗威登斯菌的最小抑菌浓度为0.25 mg/mL,与对照组相比,最小抑菌浓度剂量的丁香酚处理条件下,普罗威登斯菌菌体严重变形,出现大量的褶皱,胞内物质流出,胞外可溶性蛋白含量提高,苹果酸脱氢酶(MDH)和琥珀酸脱氢酶(SDH)酶活分别降低了33.01%,19.81%。表明丁香酚可以通过破坏普罗威登斯菌细胞壁,改变细胞膜通透性,引起细菌胞内物质泄露,以及细菌正常新陈代谢失调等作用,从而抑制普罗威登斯菌正常生长繁殖,发挥抑菌活性。

The inhibitory activity of eugenol was evaluated on Providence vermicola,which is a strain isolated from spoiled spicy crab product.The results showed that the minimum inhibitory concentration(MIC)was 0.25 mg/mL.Compared with the control group,the cells of P.vermicola treated with MIC eugenol were severely deformed,and wrinkles appeared.The cell wall of P.vermicolatreated with eugenol was destroyed,leading to the leakage of intracellular substances and higher extracellular soluble protein content.The enzyme activities of malic acid dehydrogenase(MDH)and succinate dehydrogenase(SDH)were reduced by 33.01%and 19.81%,respectively.The normal metabolic system of bacteria is dysfunctional,and thus inhibit the growth and reproduction of P.vermicola.